Development of an Automated Culture System for Laboratory Evolution. (October 2014)

• Record Type: Journal Article
• Authors: Horinouchi, Takaaki; Minamoto, Teruaki; Suzuki, Shingo; Shimizu, Hiroshi; Furusawa, Chikara
• Is Part Of: Journal of laboratory automation. Volume 19:Number 5(2014:Oct.)
• Page Start: 478
• Page End: 482
• Publication Date: 2014-10
• Subjects: laboratory evolution -- automated culture system -- Escherichia coli; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2211068214521417
• Languages: English
• ISSNs: 2211-0682

Abstract

Laboratory evolution provides phenotype–genotype mappings and quantitative analysis of selective pressures, giving important insights about evolutionary dynamics. Moreover, parallel laboratory evolution clarifies which phenotypic and genotypic changes are inevitable for adaptive evolution. Such parallel experiments, however, remain labor-intensive. In this study, to facilitate massive parallel laboratory evolution, we developed an automated culture system that can maintain hundreds of independent culture series in exponential growth phase under various culture conditions. We demonstrate the performance of this automated culture system using the laboratory evolution of Escherichia coli under various stressors.