Antigastritis effects of Armillariella tabescens (Scop.) Sing. and the identification of its anti‐inflammatory metabolites. (22nd January 2018)

• Record Type: Journal Article
• Authors: Lee, Seulah; Lee, Dahae; Park, Jun Yeon; Seok, Soonja; Jang, Tae Su; Park, Hyun Bong; Shim, Sang Hee; Kang, Ki Sung; Kim, Ki Hyun
• Is Part Of: Journal of pharmacy and pharmacology. Volume 70:Number 3(2018)
• Page Start: 404
• Page End: 412
• Publication Date: 2018-01-22
• Subjects: antigastritis -- anti‐inflammation -- Armillariella tabescens -- NF‐kappa B -- Tricholomataceae; Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12871
• Languages: English
• ISSNs: 0022-3573

Abstract

Abstract: Objectives: This study demonstrates the biological and chemical analysis of the mushroom Armillariella tabescens (Scop.) Sing. (Tricholomataceae). Methods: Chemical structures of the isolates were determined by 1D and 2D NMR, and ESI‐MS, as well as comparison with previously reported data. All isolates were tested for anti‐inflammatory effects based on their ability to inhibit LPS‐stimulated nitric oxide (NO) production in RAW264.7 cells. Key findings: We found that the MeOH extract of the fruiting bodies of A. tabescens showed antigastritis activity against ethanol‐induced gastric damage in rats and notably reduced the gastric damage index compared to control in a concentration‐dependent manner. Chemical investigation of the MeOH extract led to the isolation of four steroids (1 –4 ), three alkaloids (5 –7 ), two nucleic acids (8 –9 ) and four fatty acids (10 –13 ). This is the first study to report the identification of all isolates, except for compound7, from A. tabescens . Compounds1, 2, 3, 4 and10 showed inhibition on LPS‐stimulated NO production. Treatment with compound10 inhibited expression of iNOS, COX‐2, phospho‐IKKα, IKKα, phospho‐IκBα, IκBα and NF‐kappa B in LPS‐stimulated RAW264.7 cells. Conclusions: Compound10 likely contributes to the health benefits of A. tabescens as an antigastritis agent through its anti‐inflammatory effects.