Suppression of lung inflammation by the methanol extract of Spilanthes acmella Murray is related to differential regulation of NF-κB and Nrf2. (10th May 2018)

• Record Type: Journal Article
• Authors: Kim, Kyun Ha; Kim, Eun Jung; Kwun, Min Jung; Lee, Ji Yeon; Bach, Tran The; Eum, Sang Mi; Choi, Jun Yong; Cho, Sayeon; Kim, Sang-Jun; Jeong, Seung-Il; Joo, Myungsoo
• Is Part Of: Journal of ethnopharmacology. Volume 217(2018)
• Page Start: 89
• Page End: 97
• Publication Date: 2018-05-10
• Subjects: SAM Spilanthes acmella Murray -- PBS phosphate-buffered saline -- RT-PCR reverse transcription-polymerase chain reaction -- MPO myeloperoxidase -- LPS lipopolysaccharide -- Nrf2 nuclear erythroid 2-related factor 2 -- NF-B nuclear factor kappa-light-chain-enhancer of activated B cells; Spilanthes acmella Murray -- Anti-inflammation -- Neutrophilic lung inflammation -- Nrf2 -- NF-B; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2018.02.011
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Although Spilanthes acmella has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of S. acmella Murray (SAM). Materials and method: The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin. Results: The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2. Conclusion: Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of S. acmella . Graphical abstract: