Cytochrome‐P450‐Induced Ordering of Microsomal Membranes Modulates Affinity for Drugs. Issue 13 (22nd February 2018)

• Record Type: Journal Article
• Authors: Barnaba, Carlo; Sahoo, Bikash Ranjan; Ravula, Thirupathi; Medina‐Meza, Ilce G.; Im, Sang‐Choul; Anantharamaiah, G. M.; Waskell, Lucy; Ramamoorthy, Ayyalusamy
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 13(2018)
• Page Start: 3391
• Page End: 3395
• Publication Date: 2018-02-22
• Subjects: biophysics -- hemeproteins -- lipids -- membranes -- nanodiscs; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201713167
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Although membrane environment is known to boost drug metabolism by mammalian cytochrome P450s, the factors that stabilize the structural folding and enhance protein function are unclear. In this study, we use peptide‐based lipid nanodiscs to "trap" the lipid boundaries of microsomal cytochrome P450 2B4. We report the first evidence that CYP2B4 is able to induce the formation of raft domains in a biomimetic compound of the endoplasmic reticulum. NMR experiments were used to identify and quantitatively determine the lipids present in nanodiscs. A combination of biophysical experiments and molecular dynamics simulations revealed a sphingomyelin binding region in CYP2B4. The protein‐induced lipid raft formation increased the thermal stability of P450 and dramatically altered ligand binding kinetics of the hydrophilic ligand BHT. These results unveil membrane/protein dynamics that contribute to the delicate mechanism of redox catalysis in lipid membrane. Abstract : Redox catalysis in the lipid membrane : A novel application of peptide nanodiscs shows that cytochrome P450 2B4 is able to induce the formation of lipid raft domains in a biomimetic compound of the endoplasmic reticulum (ER). The protein‐induced lipid rafts increase the thermal stability cytochrome P450 and dramatically alter the ligand‐binding kinetics of the hydrophilic ligand BHT.