Cis-Resveratrol produces anti-inflammatory effects by inhibiting canonical and non-canonical inflammasomes in macrophages. Issue 7 (October 2014)

• Record Type: Journal Article
• Authors: Huang, Tsung-Teng; Lai, Hsin-Chih; Chen, Young-Bin; Chen, Lih-Geeng; Wu, Yi-Hui; Ko, Yun-Fei; Lu, Chia-Chen; Chang, Chih-Jung; Wu, Cheng-Yeu; Martel, Jan; Ojcius, David M; Chong, Kowit-Yu; Young, John D
• Is Part Of: Innate immunity. Volume 20:Issue 7(2014)
• Page Start: 735
• Page End: 750
• Publication Date: 2014-10
• Subjects: Resveratrol -- inflammasome -- interleukin-1β -- caspase -- reactive oxygen species; Natural immunity -- Periodicals; Endotoxins -- Periodicals; 616.07905
• DOI: 10.1177/1753425913507096
• Languages: English
• ISSNs: 1753-4259

Abstract

Resveratrol, a natural phenolic compound found in red grapes and wine, exists as cis and trans isomers. Recent studies have shown that trans -resveratrol possesses anti-inflammatory, anti-oxidant, anti-carcinogenic, anti-tumor and immunomodulatory properties. However, it remains unclear whether cis -resveratrol may exhibit similar activities. The objective of the present study was to examine the effects of cis - and trans -resveratrol on the production of pro-inflammatory cytokines and mediators in human macrophages. We examined the possibility that cis - and trans -resveratrol may affect cytokine secretion by modulating inflammasomes, intracellular multi-protein complexes, the assembly of which leads to caspase-1 activation and secretion of active IL-1β by macrophages. Our results show that pre-treatment of macrophages with cis -resveratrol not only reduces pro-IL-1β production and IL-1β secretion, but also suppresses ATP-induced transcription and activation of caspase-1 and caspase-4. Notably, cis -resveratrol inhibits the expression of the purinergic receptor, P2X7 R, and the endoplasmic reticulum stress marker, Glc-regulated protein 78, but also reduces reactive oxygen species production. Moreover, cis -resveratrol attenuates cyclooxygenase-2 expression and prostaglandin E2 production. cis -Resveratrol also decreases the phosphorylation of p38 MAPK and expression of the c-Jun protein. These results indicate that cis -resveratrol produces anti-inflammatory effects by inhibiting both the canonical and non-canonical inflammasomes, and associated pathways in human macrophages.