Evaluation of 13-week repeated oral dose toxicity of Areca catechu in F344/N rats. (April 2018)

• Record Type: Journal Article
• Authors: Kim, Hyun-Ji; Ko, Je-Won; Cha, Seung-Beom; Heo, Hyun-Suk; Seo, Jong-Hun; Cha, Mi-Jin; Bae, Jin-Sook; Lee, Hyun-Kul; Song, Si-Whan; Kim, Jong-Choon
• Is Part Of: Food and chemical toxicology. Volume 114(2018)
• Page Start: 41
• Page End: 51
• Publication Date: 2018-04
• Subjects: Subchronic toxicity -- Areca catechu water extract -- NOAEL -- Target organ; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.02.015
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: This study investigated the potential toxicity of the Areca catechu water extract after 13-week repeated oral administration at 0, 166.7, 500, and 1500 mg/kg/day in rats. During the study period, clinical signs, mortality, body weight, food consumption, water consumption, urinalysis, estrous cycle, sperm count and motility, ophthalmoscopy, hematology, serum biochemistry, gross pathology, organ weights, and histopathology were examined. At 1500 mg/kg/day, both sexes exhibited an increase in the incidence of abnormal clinical signs, which included, decreased body weight gain and food consumption, and increased urine bilirubin, ketone bodies, specific gravity, and protein and kidney weight. An increase in liver weight and estrous cycle alterations was observed in females. Serum biochemical and histopathological investigations revealed an increase in the levels of serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase, and the incidence of hepatic necrosis in females. At 500 mg/kg/day, an increase in the incidence of abnormal clinical signs including diarrhea and soiled perineal region, was observed in both sexes. No treatmentrelated effects were observed at 166.7 mg/kg/day. Under the present experimental conditions, the target organs were determined to be the liver, kidney, and female reproductive system in rats. The no-observedeffect level was considered to be 166.7 mg/kg/day in rats. Highlights: Subchronic toxicity of Areca catechu water extract was investigated in rats. The target organs of the extract were determined to be the liver, kidney, and female reproductive system. The no-observed-effect level of the extract was considered to be 166.7 mg/kg/day in rats.