Investigation of Aspergillus flavus in animal virulence. (April 2018)

• Record Type: Journal Article
• Authors: Lan, Huahui; Wu, Lianghuan; Sun, Ruilin; Yang, Kunlong; Liu, Yinghang; Wu, Jiefei; Geng, Longpo; Huang, Chuanzhong; Wang, Shihua
• Is Part Of: Toxicon. Volume 145(2018)
• Page Start: 40
• Page End: 47
• Publication Date: 2018-04
• Subjects: Aspergillosis -- Infection -- Pathogen -- Aspergillus flavus; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2018.02.043
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Aspergillus flavus is a common fungal pathogen of plants, animals and humans. Recently, many genes of A. flavus have been reported involving in regulation of pathogenesis in crops, but whether these genes are involved in animal virulence is still unknown. Here, we used a previous easy-to-use infection model for A. flavus based on mouse model by intravenous inoculation of A. flavus conidia. The outcome of infections in mice model showed that A. flavus NRRL3357 and laboratory strain CA14 PTS were both in dose dependent manner and highly reproducible. The progress of disease could be monitored by mice survival and histology analysis. Fungal burden analysis indicated it was gradually decreased within 7 days after infection. Moreover, aspergillosis caused by A. flavus significantly up-regulated gene expression levels of immune response mediators, including INF-γ, TNF-α, Dectin-1 and TLR2. Furthermore, the defined deletion A. flavus strains that previously displayed virulence in crop infection were also determined in this mouse model, and the results showed comparable degrees of infection in mice. Our results suggested that intravenous inoculation of conidia could be a suitable model for testing different A. flavus mutants in animal virulence. We hope to use this model to determine distinct A. flavus strains virulence in animals and study novel therapeutic methods to help control fungus diseases in the future. Highlights: We monitored the process of infection by survival of mice, fungal burden of the lungs and cytokine profiles. We describe the application of a murine infection model to analyze virulence of different Aspergillus flavus isolates. This infection model is suitable to test virulence and might be used to develop new therapeutics to fight fungal infections.