Simultaneous recovery of glycyrrhizic acid and liquiritin from Chinese licorice root (Glycyrrhiza uralensis Fisch) by aqueous two-phase system and evaluation biological activities of extracts. Issue 9 (13th June 2018)

• Record Type: Journal Article
• Authors: Huang, Long; Li, Wenliang; Feng, Yunchao; Fang, Xu; Li, Jiayu; Gao, Zhonghong; Li, Hailing
• Is Part Of: Separation science and technology. Volume 53:Issue 9(2018)
• Page Start: 1342
• Page End: 1350
• Publication Date: 2018-06-13
• Subjects: Aqueous two-phase system -- purification -- glycyrrhizic acid -- liquiritin -- tyrosinase -- antioxidative activity; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2018.1444052
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: Aqueous two-phase system (ATPS) was used for simultaneous purification of glycyrrhizic acid (GA) and liquiritin (LQ) from crude extract of Chinese licorice root. It was revealed that 87% GA and 94% LQ were retrieved in the ATPS top phase, under the optimum conditions of 25% (w/w) ethanol, 30% (w/w) K2 HPO4 and 4% (w/w) loading sample at 10–40°C. Compared with crude extract, the ATPS top-phase extract exhibited the highest antioxidative activity, but no tyrosinase inhibitory effect. Whereas, the ATPS bottom-phase extract was proved to be effective ABTS radical scavenger and tyrosinase inhibitor, suggesting the potency of the alcohol-salt ATPS purification for the different medicinal purposes.