LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR‐96/FOXO3. Issue 4 (2nd March 2018)

• Record Type: Journal Article
• Authors: Zhou, Yongping; Chen, Yigang; Ding, Wenzhou; Hua, Zhiyuan; Wang, Liying; Zhu, Ye; Qian, Haixin; Dai, Tu
• Is Part Of: IUBMB life. Volume 70:Issue 4(2018)
• Page Start: 276
• Page End: 290
• Publication Date: 2018-03-02
• Subjects: LncRNA UCA1 -- miR‐96 -- FOXO3 -- pancreatic cancer; Biochemistry -- Periodicals; Molecular biology -- Periodicals; 572.8
• DOI: 10.1002/iub.1699
• Languages: English
• ISSNs: 1521-6543

Abstract

Abstract: This study was expected to reveal the regulatory effects of lncRNA UCA1 on pancreatic cancer cell progression through targeting miR‐96/ FOXO3 . Microarray analysis was carried out on 36 cases of pancreatic cancer tissues and 16 cases of adjacent tissues among them. Expression levels of lncRNA UCA1, miR‐96, and FOXO3 in pancreatic cancer tissues and cell lines were determined by qRT‐PCR. Expression levels of FOXO3 protein were determined by western blot. Cell viability, cell cycle and apoptosis, cell invasion and migration were detected by CCK‐8, flow cytometry, and transwell assay, respectively. The colocalization relationship between lncRNA UCA1 and miR‐96 was detected by RNA FISH. Whether UCA1 could target miR‐96 and whether miR‐96 could target FOXO3 3′UTR were verified by dual‐luciferase reporter gene assay. High expression of lncRNA UCA1 and FOXO3 and low expression of miR‐96 were shown in pancreatic cancer. Inhibition of UCA1 suppressed pancreatic tumor cell proliferation, colony formation, and metastasis, while inhibition of miR‐96 promoted pancreatic cancer cell progression. FOXO3 was the downstream target gene of miR‐96 and showed the opposite effects. LncRNA UCA1 promoted cell proliferation, invasion, migration and inhibited cell apoptosis of pancreatic cancer through down‐regulating miR‐96 and up‐regulating FOXO3 . © 2018 IUBMB Life, 70(4):276–290, 2018