Transcriptional patterns associated with BDCA3 expression on BDCA1+ myeloid dendritic cells. Issue 3 (1st February 2018)

• Record Type: Journal Article
• Authors: van der Aa, Evelyn; Biesta, Paula J; Woltman, Andrea M; Buschow, Sonja I
• Is Part Of: Immunology and cell biology. Volume 96:Issue 3(2018)
• Page Start: 330
• Page End: 336
• Publication Date: 2018-02-01
• Subjects: BDCA3 -- dendritic cell activation -- immunology -- innate immune cells; Immunology -- Periodicals; Cytology -- Periodicals; 616.079
• DOI: 10.1111/imcb.12002
• Languages: English
• ISSNs: 0818-9641

Abstract

Abstract: Myeloid dendritic cells, including BDCA3 hi DCs and BDCA1 + DCs (hereafter dubbed DC1 and DC2 for clarity), play a pivotal role in the induction and regulation of immune responses. Interestingly, a fraction of DC2 also express low to intermediate levels of BDCA3. It is unknown whether BDCA3 + DC2 also share other traits with DC1 that are absent in BDCA3 − DC2 and/or whether BDCA3 expression renders DC2 functionally distinct from their BDCA3‐lacking counterparts. Here, we used expression analysis on a predefined set of immunology‐related genes to determine divergence between BDCA3‐positive and BDCA3‐negative DC2 and their relation to bona fide BDCA3 hi DC1. Results showed that mRNA fingerprints of BDCA3 + DC2 and BDCA3 − DC2 are very similar, and clearly distinct from that of DC1. Differences in mRNA expression, however, were observed between BDCA3 + DC2 and BDCA3 − DC2 that pointed toward a more activated status of BDCA3 + DC2. In line with this, higher steady state maturation marker expression and TLR‐induced maturation marker expression and inflammatory cytokine production by BDCA3 + DC2 were observed. This dataset provides insight into the relationship between myeloid DC populations and contributes to further understanding of DC immunobiology. Abstract : The myeloid BDCA1 + DC2 population consists of BDCA3 + and BDCA3 − cells. The divergence between BDCA3 + and BDCA3 − DC2 and their relation to bona fide BDCA3 hi DC1 was assessed by expression analysis of immunology‐related genes. Results showed that although BDCA3 + DC2 and BDCA3 − DC2 are highly comparable and distinct from DC1, BDCA3 + DC2 have a more activated phenotype than BDCA3 − DC2, and produce higher levels of cytokines upon TLR ligation.