Epigallocatechin gallate-zinc oxide co-crystalline nanoparticles as an anticancer drug that is non-toxic to normal cells. Issue 14 (15th February 2018)

• Record Type: Journal Article
• Authors: Samutprasert, Pawatsanai; Chiablaem, Khajeelak; Teeraseranee, Chanon; Phaiyarin, Punnawich; Pukfukdee, Puttikorn; Pienpinijtham, Prompong; Svasti, Jisnuson; Palaga, Tanapat; Lirdprapamongkol, Kriengsak; Wanichwecharungruang, Supason
• Is Part Of: RSC advances. Volume 8:Issue 14(2018)
• Page Start: 7369
• Page End: 7376
• Publication Date: 2018-02-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7ra10997k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A tea polyphenol, epigallocatechin-3-gallate (EGCG), can enhance cytotoxicity of Zinc in cancer cells. Here we synthesize hybrid EGCG-ZnO nanoparticles that can kill PC-3 prostate cancer cells at concentrations that are not toxic to normal cells. Abstract : Decreased uptake and cellular accumulation of zinc is a common characteristic in cancer of the liver, pancreas and prostate, because these malignant cells are intolerant to the physiological concentrations of zinc. A tea polyphenol, epigallocatechin-3-gallate (EGCG), can enhance the cytotoxicity of zinc ions to cancer, but the application of this is limited by the low stability of EGCG. In this work, we have prepared a material that can simultaneously preserve the EGCG stability and facilitate zinc uptake and accumulation in cancer cells, under conditions that are not harmful to normal cells. Thus, we co-crystallize zinc oxide with EGCG to obtain hybrid EGCG-ZnO crystalline nanoparticles of 16.5 ± 5.3 nm in diameter. The EGCG-ZnO particles effectively kill PC-3 prostate adenocarcinoma cells at concentrations that are not cytotoxic to normal cells, WI-38 human embryonic lung fibroblasts. The EGCG-ZnO particles are two times more cytotoxic against PC-3 cells than the standard ZnO particles. In PC-3 cells, the EGCG-ZnO particles are taken up by endocytosis, followed by lysosomal disruption to release zinc and EGCG into the cytoplasm, finally resulting in nuclear accumulation of zinc.