Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds. (October 2014)

• Record Type: Journal Article
• Authors: Benders, K.E.M.; Boot, W.; Cokelaere, S.M.; Van Weeren, P.R.; Gawlitta, D.; Bergman, H.J.; Saris, D.B.F.; Dhert, W.J.A.; Malda, J.
• Is Part Of: Cartilage. Volume 5:Number 4(2014)
• Page Start: 221
• Page End: 230
• Publication Date: 2014-10
• Subjects: cartilage tissue engineering -- chondrocyte -- extracellular matrix (ECM) -- mesenchymal stromal cell -- scaffold; Cartilage -- Periodicals; 616.7
• DOI: 10.1177/1947603514535245
• Languages: English
• ISSNs: 1947-6035

Abstract

Objective . Although extracellular matrix (ECM)–derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro. Design . Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content). Results . After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix. Conclusion . These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.