99mTc‐labeled rHuEpo for imaging of the erythropoietin receptor in tumors. (2nd February 2018)

• Record Type: Journal Article
• Authors: Zhuang, Xiaoqing; Zhao, Dan; Yang, Pengfei; Jia, Yingqin; Liang, Rui; Zhao, Qian; Han, Chunlei; Kinsella, Joseph M.; Sheng, Ruilong; Li, Juan
• Is Part Of: Journal of labelled compounds & radiopharmaceuticals. Volume 61:Number 2(2018)
• Page Start: 77
• Page End: 83
• Publication Date: 2018-02-02
• Subjects: 99mTc -- erythropoietin receptor -- molecular imaging -- radiotracer -- rHuEpo; Tracers (Chemistry) -- Periodicals; Radiopharmaceuticals -- Periodicals; 615.8424
• DOI: 10.1002/jlcr.3586
• Languages: English
• ISSNs: 0362-4803

Abstract

Abstract : To analyze erythropoietin receptor (EpoR) status in tumors, recombinant human erythropoietin (rHuEpo) was labeled with 99m Tc by 99m Tc‐centered 1‐pot synthesis, resulting in high radiochemical purity, stability, and biological activity. Both in vitro cell culture experiments and biodistribution studies of normal rats demonstrated successful EpoR targeting. The biodistribution of labeled rHuEpo in a NCI‐H1975 xenograft model showed tumor accumulation (tumor‐to‐muscle ratio, 4.27 ± 1.77), confirming the expression of active EpoR in tumors. Thus, as a novel single positron emission computerized tomography tracer for the imaging of EpoR expression in vivo, 99m Tc‐rHuEpo is effective for exploring the role of EpoR in cancer growth, metastasis and angiogenesis. Abstract : To analyze erythropoietin receptor (EpoR) status in tumor, 99m Tc‐recombinant human erythropoietin ( 99m Tc‐rHuEpo) was synthesized, and the EpoR targeting was confirmed by cell study and rat biodistribution study. The biodistribution of NCI‐H1975 xenografts model showed tumor accumulation, which confirmed the expression of active EpoR in tumors. Thus, as a novel SPECT tracer for imaging of EpoR expression in vivo, 99m Tc‐rHuEpo was useful to explore the role of EpoR in cancer growth, metastasis, and angiogenesis.