Multifunctional Poly‐N‐Isopropylacrylamide/DNAzyme Microgels as Highly Efficient and Recyclable Catalysts for Biosensing. (10th January 2018) - Li, Fengyun
Abstract: The development of highly efficient, recyclable, and multifunctional biocatalysts is of great importance for various applications, especially in biosensing. In this study, highly catalytic and recyclable DNAzyme functionalized poly‐ N ‐isopropylacrylamide (pNIPAM) microgels are prepared via one‐step precipitation polymerization. The pNIPAM/DNAzyme microgels exhibit highly catalytic activities in aqueous solution at room temperature, and become hydrophobic and separable from the reaction mixture at temperature higher than the lower critical solution temperature of pNIPAM, which facilitate the recyclable utilization of these catalysts. Different kinds of DNAzyme functionalized catalytic microgels can be facilely prepared via the one‐step synthesis procedure. Two typical catalytic DNA structures, the Mg 2+ ‐dependent DNAzyme and the hemin‐G‐quadruplex horseradish peroxidase (HRP)‐mimicking DNAzyme, are chosen as model systems to validate the feasibility. These pNIPAM/DNAzyme microgel catalysts maintain 80% to 91% initial catalytic activity after eight times of catalysis recycling. Furthermore, the pNIPAM microgels by themselves provide additional interfaces to capturing an enzyme, glucose oxidase, which can cascade with the linked HRP mimicking DNAzymes, to form recyclable bi‐enzyme cascading system for the sensing of glucose. Abstract : Synthesized by one‐step precipitation polymerization, multifunctional poly‐ N ‐isopropylacrylamide (pNIPAM)/DNAzyme microgels exhibit high catalytic activities at room temperature, and can be easily separated from the reaction mixture by heating–centrifuging processes, which facilitates the recycled utilization of these biocatalysts. The pNIPAM microgels provide additional interfaces to capture enzymes, which can cascade with the linked DNAzymes, to form recyclable cascading systems for biosensing.