Transport of valine across the small intestinal epithelium in pigs fed different valine levels and Bacillus subtilis. Issue 2 (28th November 2017)

• Record Type: Journal Article
• Authors: Blaabjerg, K.; Nørgaard, J. V.; Nielsen, B.; Cantor, M. D.; Derkx, P.; Sehested, J.; Poulsen, H. D.
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 102:Issue 2(2018)
• Page Start: e856
• Page End: e863
• Publication Date: 2017-11-28
• Subjects: absorption -- amino acids -- Bacillus subtilis -- pigs -- Ussing chamber -- valine transport; Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/jpn.12846
• Languages: English
• ISSNs: 0931-2439

Abstract

Summary: Mutants of Bacillus subtilis overproducing valine ( B. subtilis VAL) could be an approach to supply pigs dietary valine (Val). In the study, 18 gilts were fed: (i) negative diet with a standardized ileal digestible (SID) Val:Lys of 0.63:1 (Neg); (ii) Neg added B. subtilis VAL (1.28 × 10 11 cfu/kg as‐fed) or; (iii) Neg added L‐Val to a Val:Lys of 0.69:1. Using the Ussing chamber method, the study aimed to investigate whether (i) the diets affect intestinal transport of additions of 0, 5, 10 or 20 mmol Val/L from the mucosal to the serosal side and (ii) the B. subtilis VAL contributes to a net transport of Val produced in situ. The results showed that the Isc (ΔIscVal ) and release of Val to the serosal side solution (Srel ; μmol cm −2 min −1 ) increased with Val addition (linear and quadratic, p < .0001) but was similar for 5, 10 or 20 mmol Val/L and not affected by diet. No net transport of in situ produced Val by B. subtilis VAL was detected. In conclusion, feeding a Val‐deficient diet with or without B. subtilis VAL or a Val sufficient diet did not affect the Val transport across intestinal epithelia. No in situ Val production by B. subtilis VAL was observed in the Ussing chambers.