The Evaluation of IL6 and ESR1 Gene Polymorphisms in Primary Dysmenorrhea. (2nd January 2016)

• Record Type: Journal Article
• Authors: Ozsoy, Asker Zeki; Karakus, Nevin; Yigit, Serbulent; Cakmak, Bulent; Nacar, Mehmet Can; Yılmaz Dogru, Hatice
• Is Part Of: Immunological investigations. Volume 45:Number 1(2016)
• Page Start: 75
• Page End: 86
• Publication Date: 2016-01-02
• Subjects: ESR1 -- genetic susceptibility -- IL6 -- polymorphism -- primary dysmenorrhea; Immunology -- Periodicals; Immunochemistry -- Periodicals; Cellular immunity -- Periodicals; Communicable diseases -- Periodicals; 616.079
• DOI: 10.3109/08820139.2015.1113426
• Languages: English
• ISSNs: 0882-0139

Abstract

ABSTRACT: Primary dysmenorrhea is the most common gynecological complaint with painful menstrual cramps in pelvis without any pathology. It affects about half of menstruating women, and it causes significant disruption in quality of life. We investigated the association between IL6 gene promoter and ESR1 gene Xba I and Pvu II polymorphisms and primary dysmenorrhea. In this case–control study, 152 unrelated young women with primary dysmenorrhea and 150 unrelated healthy age-matched controls participated. Genomic DNA was isolated and IL6 and ESR1 gene polymorphisms were genotyped using PCR-based RFLP assay. The distribution of genotype and allele frequencies of IL6 gene promoter and ESR1 gene Xba I polymorphisms were not statistically different between patients and controls ( p > 0.05). However, the genotype and allele frequencies of ESR1 gene Pvu II polymorphism showed statistically significant differences between primary dysmenorrhea patients and controls ( p = 0.009 and p = 0.021, respectively). Statistically significant associations were also observed between age and married status of primary dysmenorrhea patients and ESR1 gene Pvu II polymorphism ( p = 0.044 and p = 0.023, respectively). In combined genotype analyses, AG at ESR1 Xba I and TC at ESR1 Pvu II loci encoded a p -value of 0.027. Thus, individuals who are heterozygote at both loci have a lower risk of developing primary dysmenorrhea. Our study suggests no strong association between IL6 gene promoter and ESR1 gene Xba I polymorphisms and primary dysmenorrhea in Turkish women. However, ESR1 gene Pvu II polymorphism showed statistically significant differences between primary dysmenorrhea patients and controls. The potential association between ESR1 gene Pvu II polymorphism and age and married status of dysmenorrhea patients deserves further consideration.