Competitive immunoassay for monitoring polybrominated diphenyl ethers in PM2.5 in Shanghai. Issue 2 (26th January 2018)

• Record Type: Journal Article
• Authors: Zhang, Xiaohan; Zhuang, Huisheng; Ma, Hui; Ping, Xianyin; Ding, Qian
• Is Part Of: International journal of environmental analytical chemistry. Volume 98:Issue 2(2018)
• Page Start: 97
• Page End: 110
• Publication Date: 2018-01-26
• Subjects: Immunoassay -- PBDEs -- PM2.5 -- air monitoring -- gaseous pollutants; Ecology -- Periodicals; Chemistry, Analytic -- Periodicals; Environmental chemistry -- Periodicals; Pollution -- Periodicals; 577.14
• DOI: 10.1080/03067319.2018.1434167
• Languages: English
• ISSNs: 0306-7319

Abstract

ABSTRACT: In this paper, a sensitive biotin–streptavidin (BS)-ELISA was developed for determining the polybrominated diphenyl ethers in PM2.5 . For establishing this proposed BS-ELISA, we prepared the biotinylated antibody primarily. And for reducing the background interference, some influencing factors and procedures for this immunoassay were also discussed and optimised. Under the optimal conditions, the IC50 = 0.53 ng/mL; IC10 was 0.002 ng/mL; and the results were almost consistent with those using the gas chromatography and electron capture detection (GC-ECD). Less procedures and simpler sample preparation were required for this method compared with the GC-ECD. The results showed that the highest value of BDE-47 concentration occurred in December, which might reflect the combination of heating and industrial pollution. In our analysis, we studied the Pearson correlations between BDE-47/PM2.5 and gaseous pollutants (such as NO2, SO2, CO, O3, PM10 and PM2.5 ). BDE-47 showed a higher correlation with NO2 than that with PM10, PM2.5 and SO2, which implied that the BDE-47 emission process might be accompanied by the emission of NO2 . Moreover, during the Spring Festival, the concentration of BDE-47 in PM2.5 decreased significantly, whereas the PM2.5 changed little. This suggested factories and vehicles might be the major contributors to BDE-47 emissions (but not to PM2.5 ).