Microarray Analysis of Human Blood During Electroconvulsive Therapy. Issue 4 (December 2015)

• Record Type: Journal Article
• Authors: Kaneko, Takao; Kanazawa, Tetsufumi; Nishiguchi, Masaki; Kikuyama, Hiroki; Tsutsumi, Atsushi; Uenishi, Hiroyuki; Kawabata, Yasuo; Kawashige, Seiya; Nishizawa, Yoshitaka; Maruyama, Souichiro; Koh, Jun; Yoneda, Hiroshi
• Is Part Of: Journal of ECT. Volume 31:Issue 4(2015:Dec.)
• Publication Date: 2015-12
• Subjects: modified electroconvulsive therapy -- mECT -- microarray -- catatonic schizophrenia -- quantitative polymerase chain reaction -- qPCR -- AKT3 -- TCF7 -- PPP3R1 -- GADD45B -- mRNA -- gene expression; Electroconvulsive therapy -- Periodicals; Shock therapy -- Periodicals; Electroconvulsive Therapy -- Periodicals; Electroconvulsive therapy; Shock therapy; Periodicals; Periodicals; Electronic journals; 616.89122
• DOI: 10.1097/YCT.0000000000000234
• Languages: English
• ISSNs: 1095-0680

Abstract

Abstract : Introduction: Electroconvulsive therapy (ECT) is currently regarded as a significant treatment option for intractable psychiatric disorders, such as catatonic schizophrenia or treatment-resistant depression; however, the underlying molecular mechanism for its therapeutic effect remains obscure. Methods: Employing microarray analysis (Human Genome U133 Plus 2.0 Array; Affymetrix, United States) of cDNA derived from the peripheral blood of patients with catatonic schizophrenia (n = 5), we detected a significant change in 145 genes (0.68%) before and after modified ECT (mECT). Moreover, we performed quantitative polymerase chain reaction validation of genes that had previously been suggested to be functionally related to schizophrenia. Results: Of 4 genes examined ( AKT3, TCF7, PPP3R1, and GADD45B ), only TCF7 was increased during the mECT procedure ( P = 0.0025). Discussion: This study describes the first attempt to uncover the molecular mechanism of mECT using a microarray assay of mRNA derived from peripheral blood, and our results suggest that the TCF family may play a role in the functional mechanism of mECT.