Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels. Issue 3 (30th January 2018)

• Record Type: Journal Article
• Authors: Lampard, Emma V.; Sedgwick, Adam C.; Sombuttan, Thitima; Williams, George T.; Wannalerse, Boontana; Jenkins, A. Toby A.; Bull, Steven D.; James, Tony D.
• Is Part Of: ChemistryOpen. Volume 7:Issue 3(2018)
• Page Start: 266
• Page End: 268
• Publication Date: 2018-01-30
• Subjects: boronic acids -- colorimetric detection -- dye displacement assays -- glucose -- saccharides; Chemistry -- Periodicals; 540; 540.5
• DOI: 10.1002/open.201700193
• Languages: English
• ISSNs: 2191-1363

Abstract

Abstract: Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose. Abstract : BOB's your uncle : Benzoxaborole (BOB) hydrogels are developed and are shown to have an enhanced binding affinity towards monosaccharides, in particular towards glucose.