BMP signaling and microtubule organization regulate synaptic strength. (16th April 2015)

• Record Type: Journal Article
• Authors: Ball, R.W.; Peled, E.S.; Guerrero, G.; Isacoff, E.Y.
• Is Part Of: Neuroscience. Volume 291(2015)
• Page Start: 155
• Page End: 166
• Publication Date: 2015-04-16
• Subjects: BMP Bone Morphogenetic Protein -- NMJ neuromuscular junction -- VGlut vesicular glutamate transporter -- ΔFRET change in fluorescence resonance energy transfer; drosophila -- neuromuscular junction -- synaptic transmission -- Bone Morphogenetic Protein signaling pathway -- microtubule -- fluorescent calcium sensor; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2015.01.069
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Synaptic transmission strength was imaged using a postsynaptic calcium indicator. The proximal–distal gradient of synaptic strength is disrupted in BMP mutants. Unbundled MAP1B, found in loops, is associated with stronger boutons. Alterations in microtubule structure impair the synaptic transmission gradient. Abstract: The strength of synaptic transmission between a neuron and multiple postsynaptic partners can vary considerably. We have studied synaptic heterogeneity using the glutamatergic Drosophila neuromuscular junction (NMJ), which contains multiple synaptic connections of varying strengths between a motor axon and muscle fiber. In larval NMJs, there is a gradient of synaptic transmission from weak proximal to strong distal boutons. We imaged synaptic transmission with the postsynaptically targeted fluorescent calcium sensor SynapCam, to investigate the molecular pathways that determine synaptic strength and set up this gradient. We discovered that mutations in the Bone Morphogenetic Protein (BMP) signaling pathway disrupt production of strong distal boutons. We find that strong connections contain unbundled microtubules in the boutons, suggesting a role for microtubule organization in transmission strength. The spastin mutation, which disorganizes microtubules, disrupted the transmission gradient, supporting this interpretation. We propose that the BMP pathway, shown previously to function in the homeostatic regulation of synaptic growth, also boosts synaptic transmission in a spatially selective manner that depends on the microtubule system.