The effect of mild stress stimulation on the nerve growth factor (NGF) and tyrosine kinase receptor A (TrkA) immunoreactivity in the paraventricular nucleus (PVN) of the hypothalamus and hippocampus in aged vs. adult rats. (2nd April 2015)

• Record Type: Journal Article
• Authors: Badowska-Szalewska, E.; Krawczyk, R.; Ludkiewicz, B.; Moryś, J.
• Is Part Of: Neuroscience. Volume 290(2015)
• Page Start: 346
• Page End: 356
• Publication Date: 2015-04-02
• Subjects: DG dentate gyrus -- FS forced swim -- HL-OF high-light open-field -- HPA hypothalamic–pituitary–adrenal -- NGF nerve growth factor -- PVN paraventricular nucleus -- TrkA tyrosine kinase receptor A; nerve growth factor (NGF) -- tyrosine kinase receptor A (TrkA) -- stress -- aging -- limbic system; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2015.01.052
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: We studied the effect of stressors on nerve growth factor (NGF) and tyrosine kinase receptor A (TrkA) immunoreactivity. Adult and aged rats were used for acute and chronic stress experiments. Forced swim stressor did not influence NGF and TrkA changes in hypothalamic PVN and hippocampus. Acute high-light open-field stressor led to an increase in NGF in hippocampal CA1 and CA3 of both age groups. Age-related decrease in NGF and/or TrkA in stressed rats was noted. Abstract: Ontogenetic life and stress can have different effects on the nerve growth factor (NGF) and its tyrosine kinase receptor A (TrkA) in the structures of the limbic system. This study aimed to explore the influence of two different stressors, acute and chronic exposure to forced swim (FS) stress or high-light open-field (HL-OF) stress, on cells containing NGF and TrkA. Immunofluorescence staining was used to reveal the density of NGF and TrkA immunoreactive (ir) cells in the paraventricular nucleus (PVN) of the hypothalamus or hippocampal subfields CA1, CA3 and dentate gyrus (DG) in adult (postnatal day 90; P90) and aged (P720) rats. Data revealed that neither acute nor chronic FS caused any alteration in NGF-ir and TrkA-ir cells in any of the structures investigated in P90 and P720 rats. However, a significant increase in NGF-ir was detected in the CA1 and CA3 after acute but not after chronic HL-OF in both age groups. The TrkA-ir remained unchanged after exposure to HL-OF in the PVN and hippocampus. Despite lack of change in the density of NGF-ir and TrkA-ir cells between P90 and P720 non-stressed rats, a significant age-related decrease in NGF-ir and TrkA-ir cells in the PVN of FS- and HL-OF-stressed rats was noted. However, in the hippocampus, an age-related decrease in NGF-ir or TrkA-ir cells was observed in all rats except acute FS-stressed rats. The changes are possibly associated with involutional aging processes caused by insufficient control of hypothalamic–pituitary–adrenal (HPA) axis functioning in P720 rats and may contribute to disturbances in NGF signaling.