GABAergic somatostatin-immunoreactive neurons in the amygdala project to the entorhinal cortex. (2nd April 2015)

• Record Type: Journal Article
• Authors: McDonald, A.J.; Zaric, V.
• Is Part Of: Neuroscience. Volume 290(2015)
• Page Start: 227
• Page End: 242
• Publication Date: 2015-04-02
• Subjects: AHA amygdalohippocampal area -- BLa anterior basolateral nucleus -- CBL corticobasolateral amygdala -- CCK cholecystokinin -- DLEA dorsolateral entorhinal area -- FG Fluorogold -- GAD glutamic acid decarboxylase -- IN intercalated nuclei -- Lv ventromedial lateral nucleus -- LRNP long-range nonpyramidal -- M2R m2 muscarinic cholinergic receptor -- Mpd posterodorsal medial nucleus -- mPFC medial prefrontal cortex -- NPY neuropeptide Y -- PaS parasubiculum -- PBS phosphate-buffered saline -- PV parvalbumin -- SOM somatostatin -- TLE temporal lobe epilepsy -- VIP vasoactive intestinal peptide -- VLEA ventrolateral entorhinal area -- VMEA ventromedial entorhinal area; retrograde tract tracing -- long-range GABAergic neurons -- parasubiculum -- entorhinal cortex -- nonpyramidal neurons -- prefrontal cortex; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2015.01.028
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Most amygdalar neurons labeled by Fluorogold injections into the entorhinal cortex are located in the basolateral amygdala. About 1–5% of these amygdalar projection neurons are nonpyramidal neurons containing somatostatin and/or neuropeptide Y. Slightly more than half of these long-range nonpyramidal neurons (LRNP neurons) express GAD or the m2 muscarinic receptor. No LRNP neurons with entorhinal projections express parvalbumin or vasoactive intestinal peptide. No amygdalar LRNP neurons project to the prefrontal cortex. Abstract: The entorhinal cortex and other hippocampal and parahippocampal cortices are interconnected by a small number of GABAergic nonpyramidal neurons in addition to glutamatergic pyramidal cells. Since the cortical and basolateral amygdalar nuclei have cortex-like cell types and have robust projections to the entorhinal cortex, we hypothesized that a small number of amygdalar GABAergic nonpyramidal neurons might participate in amygdalo-entorhinal projections. To test this hypothesis we combined Fluorogold (FG) retrograde tract tracing with immunohistochemistry for the amygdalar nonpyramidal cell markers glutamic acid decarboxylase (GAD), parvalbumin (PV), somatostatin (SOM), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), and the m2 muscarinic cholinergic receptor (M2R). Injections of FG into the rat entorhinal cortex labeled numerous neurons that were mainly located in the cortical and basolateral nuclei of the amygdala. Although most of these amygdalar FG+ neurons labeled by entorhinal injections were large pyramidal cells, 1–5% were smaller long-range nonpyramidal neurons (LRNP neurons) that expressed SOM, or both SOM and NPY. No amygdalar FG+ neurons in these cases were PV+ or VIP+. Cell counts revealed that LRNP neurons labeled by injections into the entorhinal cortex constituted about 10–20% of the total SOM+ population, and 20–40% of the total NPY population in portions of the lateral amygdalar nucleus that exhibited a high density of FG+ neurons. Sixty-two percent of amygdalar FG+/SOM+ neurons were GAD+, and 51% were M2R+. Since GABAergic projection neurons typically have low perikaryal levels of GABAergic markers, it is actually possible that most or all of the amygdalar LRNP neurons are GABAergic. Like GABAergic LRNP neurons in hippocampal/parahippocampal regions, amygdalar LRNP neurons that project to the entorhinal cortex are most likely involved in synchronizing oscillatory activity between the two regions. These oscillations could entrain synchronous firing of amygdalar and entorhinal pyramidal neurons, thus facilitating functional interactions between them, including synaptic plasticity.