Inhibitory effect of naringenin via IL‐13 level regulation on thymic stromal lymphopoietin‐induced inflammatory reactions. (29th November 2017)

• Record Type: Journal Article
• Authors: Han, Na‐Ra; Moon, Phil‐Dong; Ryu, Ka‐Jung; Kim, Na‐Rae; Kim, Hyung‐Min; Jeong, Hyun‐Ja
• Is Part Of: Clinical and experimental pharmacology and physiology. Volume 45:Number 4(2018)
• Page Start: 362
• Page End: 369
• Publication Date: 2017-11-29
• Subjects: inflammation -- mast cells -- naringenin -- proliferation -- thymic stromal lymphopoietin; Clinical pharmacology -- Periodicals; Pharmacology, Experimental -- Periodicals; Physiology, Experimental -- Periodicals; Physiology, Pathological -- Periodicals; 615.1
• DOI: 10.1111/1440-1681.12880
• Languages: English
• ISSNs: 0305-1870

Abstract

Summary: Naringenin (NG) has various beneficial properties, such as anti‐cancer and anti‐inflammatory effects. Thymic stromal lymphopoietin (TSLP) induces mast cell proliferation and inflammatory reactions. The aim of this study was to investigate the regulatory effect of NG on TSLP‐induced mast cell proliferation and inflammatory reactions using human mast cell line (HMC‐1) cells. HMC‐1 cells were pre‐treated with NG and then treated with TSLP. HMC‐1 cells proliferation was determined by quantifying bromodeoxyuridine incorporation. Levels of anti‐apoptotic and pro‐apoptotic factors were analyzed by western blot analysis. The productions and mRNA expressions of interleukin (IL)‐13 and tumour necrosis factor‐α (TNF‐α) were analyzed by ELISA and quantitative real‐time PCR. We found that NG significantly attenuated HMC‐1 cells proliferation and Ki‐67 mRNA expression promoted by TSLP. NG significantly suppressed mRNA expression of TSLP receptor and IL‐7 receptor α in TSLP‐treated HMC‐1 cells. NG significantly down‐regulated levels of phosphorylated‐signal transducer and activation of transcription 6 and murine double‐minute 2 in TSLP‐treated HMC‐1 cells, up‐regulated levels of cleaved poly ADP‐ribose polymerase and p53 in TSLP‐treated HMC‐1 cells. Furthermore, NG significantly decreased the productions and mRNA expressions of IL‐13 and TNF‐α in TSLP‐treated HMC‐1 cells. These results suggest NG has an inhibitory effect on mast cell‐mediated allergic inflammatory reactions.