Determination of tubuloside B by LC‐MS/MS and its application to a pharmacokinetic study in rats. (6th December 2017)

• Record Type: Journal Article
• Authors: Yang, Shenbao; Qu, Ruiying; Sun, Peilu; Xiong, Shan; Yan, Siyi; Deng, Zhipeng
• Is Part Of: Biomedical chromatography. Volume 32:Number 4(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-12-06
• Subjects: tubuloside B -- liquid chromatography–tandem mass spectrometry -- pharmacokinetic study; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4138
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Tubuloside B, a novel neuroprotective phenylethanoid, is a major active constituent of Cistanche tubulosa and Cistanche deserticola . A specific and sensitive liquid chromatography tandem mass spectrometry (LC‐MS/MS) method has been developed and validated for the quantification of tubuloside B in rat plasma. Sample preparation was conducted through a protein‐precipitation extraction with methanol using tubuloside A as internal standard (IS). Chromatographic separation was achieved using a Capcell Pak C18 column (2.0 × 50 mm, 5 μm) with a mobile phase of methanol–10 mm ammonium acetate buffer (70:30, v/v) in an isocratic elution. Mass spectrometry analysis was performed in negative ionization mode with selected reaction monitoring transitions at m/z 665.1 → 160.9 for tubuloside B, and m/z 827.1 → 160.9 for IS. Calibration curves were linear over the range of 1.64–1640 ng/mL for plasma samples samples ( R 2 > 0.990). The lower limit of quantification (LLOQ) was 1.64 ng/mL. The intra‐ and inter‐day accuracy was between 92.3 and 113.0% with the RSD <9.23% at all LLOQ and quality control levels. Finally, this method was successfully applied in the pharmacokinetics study of tubuloside B after intravenous administration.