IgG from atopic dermatitis patients induces IL‐17 and IL‐10 production in infant intrathymic TCD4 and TCD8 cells. (22nd January 2018)

• Record Type: Journal Article
• Authors: Sgnotto, Fábio D. R.; de Oliveira, Marilia G.; Lira, Aline A. L.; Inoue, Amanda H. S.; Titz, Tiago O.; Orfali, Raquel L.; Bento‐de‐Souza, Luciana; Sato, Maria N.; Aoki, Valeria; Duarte, Alberto J. S.; Victor, Jefferson R.
• Is Part Of: International journal of dermatology. Volume 57:Number 4(2018)
• Page Start: 434
• Page End: 440
• Publication Date: 2018-01-22
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/ijd.13907
• Languages: English
• ISSNs: 0011-9059

Abstract

Abstract: Introduction: Our group recently demonstrated that IgG modulates αβ T cell cytokine production during the maturation process in the human thymus. The effects of this modulation are IgG repertoire dependent and can exert a systemic and long‐term impact. Objective: To investigate whether IgG from atopic dermatitis (AD) patients can modulate cytokine production of infant intrathymic TCD4 and TCD8 cells in vitro . Methods: Thymic tissues were obtained from newborn children from nonatopic mothers, and thymocytes were cultured for 6 days with purified IgG from AD patients or with intravenous immunoglobulin (IVIG) or mock conditions as controls. Cells were gated as double positive T cells (TDP − CD4 + CD8 + ), TCD4 cells (CD4 + CD8 − ), or TCD8 cells (CD4 − CD8 + ), and intracellular levels of IL‐17A, IFN‐ γ, TNF‐ α, IL‐4, IL‐10, and TGF‐ β were evaluated by flow cytometry. Results: Compared to mock and IVIG culture conditions, IgG of AD individuals induced in vitro intracellular production of IL‐17 and IL‐10 by intrathymic TDP, TCD4, and TCD8 cells of infants. TGF‐ β was also detected at a higher frequency in response to AD IgG in TDP and TCD8 cells compared to mock and IVIG cultured conditions. An opposite effect was detected upon IFN‐ γ production in TCD4 cells, such that AD IgG reduced IFN‐ γ production compared to production under mock conditions but not under IVIG conditions. Conclusion: IgG of AD patients can stimulate cytokine production in infant thymocytes and thus resembles the peripheral profile observed in adults. These findings suggest a novel mechanism that can contribute to AD pathogenesis.