Cross-generational trans fat intake facilitates mania-like behavior: Oxidative and molecular markers in brain cortex. (12th February 2015)

• Record Type: Journal Article
• Authors: Trevizol, F.; Roversi, Kr.; Dias, V.T.; Roversi, K.; Barcelos, R.C.S.; Kuhn, F.T.; Pase, C.S.; Golombieski, R.; Veit, J.C.; Piccolo, J.; Pochmann, D.; Porciúncula, L.O.; Emanuelli, T.; Rocha, J.B.T.; Bürger, M.E.
• Is Part Of: Neuroscience. Volume 286(2015)
• Page Start: 353
• Page End: 363
• Publication Date: 2015-02-12
• Subjects: AA arachidonic acid -- AMPH amphetamine -- BD bipolar disorder -- BDNF brain-derived neurotrophic factor -- DAT dopamine transporter -- DCF dichlorofluorescein -- DCHF-DA 2′, 7′-dichlorofluorescein diacetate -- DHA docosahexaenoic acid -- DNPH dinitrophenylhydrazine -- EFAs essential fatty acids -- FO fish oil -- HVF hydrogenated vegetable fat -- OS oxidative stress -- PC protein carbonyl -- PGE3 prostaglandins of series 3 -- PUFA polyunsaturated fatty acids -- PVDF polyvinylidene difluoride -- RS reactive species -- RT-PCR real-time polymerase chain reaction -- TBS Tris-buffered saline -- TFA Trans fatty acid -- SO soybean oil; trans fat -- oxidative damage -- DAT -- BDNF -- animal model of mania -- bipolar disorder; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2014.11.059
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Rats were supplemented with trans fat (TF) during two sequential generations. TF supplementation facilitated mania-behavior induced by amphetamine (AMPH) in rats. Trans fatty acid (TFA) was incorporated in the brain cortex causing oxidative damages. Dopamine transporter immune content and BDNF-mRNA levels were reduced by TF after AMPH. Processed foods contribute to develop neuropsychiatric conditions such as mania. Abstract: Since that fast food consumption have raised concerns about people's health, we evaluated the influence of trans fat consumption on behavioral, biochemical and molecular changes in the brain-cortex of second generation rats exposed to a model of mania. Two successive generations of female rats were supplemented with soybean oil (SO, rich in n-6 FA, control group), fish oil (FO, rich in n-3 FA) and hydrogenated vegetable fat (HVF, rich in trans FA) from pregnancy, lactation to adulthood, when male rats from 2nd generation received amphetamine (AMPH-4 mg/kg-i.p., once a day, for 14 days) treatment. AMPH increased locomotor index in all animals, which was higher in the HVF group. While the FO group showed increased n-3 polyunsaturated fatty acid (PUFA) incorporation and reduced n-6/n-3 PUFA ratio, HVF allowed trans fatty acid (TFA) incorporation and increased n-6/n-3 PUFA ratio in the brain-cortex. In fact, the FO group showed minor AMPH-induced hyperactivity, decreased reactive species (RS) generation per se, causing no changes in protein carbonyl (PC) levels and dopamine transporter (DAT). FO supplementation showed molecular changes, since proBDNF was increased per se and reduced by AMPH, decreasing the brain-derived neurotrophic factor (BDNF) level following drug treatment. Conversely, HVF was related to increased hyperactivity, higher PC level per se and higher AMPH-induced PC level, reflecting on DAT, whose levels were decreased per se as well as in AMPH-treated groups. In addition, while HVF increased BDNF-mRNA per se, AMPH reduced this value, acting on BDNF, whose level was lower in the same AMPH-treated experimental group. ProBDNF level was influenced by HVF supplementation, but it was not sufficient to modify BDNF level. These findings reinforce that prolonged consumption of trans fat allows TFA incorporation in the cortex, facilitating hyperactive behavior, oxidative damages and molecular changes. Our study is a warning about cross-generational consumption of processed food, since high trans fat may facilitate the development of neuropsychiatric conditions, including bipolar disorder (BD).