A rapid T1 mapping method for assessment of murine kidney viability using dynamic manganese‐enhanced magnetic resonance imaging. Issue 1 (28th November 2017)

• Record Type: Journal Article
• Authors: Jiang, Kai; Tang, Hui; Mishra, Prasanna K.; Macura, Slobodan I.; Lerman, Lilach O.
• Is Part Of: Magnetic resonance in medicine. Volume 80:Issue 1(2018)
• Page Start: 190
• Page End: 199
• Publication Date: 2017-11-28
• Subjects: manganese‐enhanced MRI -- T1 mapping -- kidney viability -- renal artery stenosis; Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.27025
• Languages: English
• ISSNs: 0740-3194

Abstract

Abstract : Purpose: Dynamic manganese‐enhanced MRI (MEMRI) allows assessment of tissue viability by tracing manganese uptake. We aimed to develop a rapid T1 mapping method for dynamic MEMRI to facilitate assessments of murine kidney viability. Methods: A multi‐slice saturation recovery fast spin echo (MSRFSE) was developed, validated, and subsequently applied in dynamic MEMRI at 16.4T on ischemic mouse kidneys after 4 weeks of unilateral renal artery stenosis (RAS). Baseline T1 values and post‐contrast R1 (1/T1 ) changes were measured in cortex (CO), outer (OSOM), inner (ISOM) strips of outer medulla, and inner medulla (IM). Results: Validation studies showed strong agreement between MSRFSE and an established saturation recovery Look‐Locker method. Baseline T1 (s) increased in the stenotic kidney CO (2.10 [1.95–2.56] vs. 1.88 [1.81–2.00], P = 0.0317) and OSOM (2.17 [2.05–2.33] vs. 1.96 [1.87–2.00], P = 0.0075) but remained unchanged in ISOM and IM. This method allowed a temporal resolution of 1.43 min in dynamic MEMRI. Mn 2+ uptake and retention decreased in stenotic kidneys, particularly in the OSOM (ΔR1 : 0.48 [0.38–0.56] vs. 0.64 [0.61–0.69] s −1, P < 0.0001). Conclusion: Dynamic MEMRI by MSRFSE detected decreased cellular viability and discerned the regional responses to RAS. This technique may provide a valuable tool for noninvasive evaluation of renal viability. Magn Reson Med 80:190–199, 2018. © 2017 International Society for Magnetic Resonance in Medicine.