Ultrastructural changes and programmed cell death of trophocytes in the gonad of Isohypsibius granulifer granulifer Thulin, 1928 (Tardigrada, Eutardigrada, Isohypsibiidae). (March 2015)

• Record Type: Journal Article
• Authors: Poprawa, Izabela; Hyra, Marta; Kszuk-Jendrysik, Michalina; Rost-Roszkowska, Magdalena Maria
• Is Part Of: Micron. Volume 70(2015:Mar.)
• Page Start: 26
• Page End: 33
• Publication Date: 2015-03
• Subjects: Oogenesis -- Nurse cell -- Cytoplasmic bridges -- Apoptosis -- Autophagy; Microscopy -- Periodicals; Electron Probe Microanalysis -- Periodicals; Microscopy -- Periodicals; Microscopie -- Périodiques; Microscopy; Periodicals; 502.82
• DOI: 10.1016/j.micron.2014.11.008
• Languages: English
• ISSNs: 0968-4328

Abstract

Highlights: The germ cell cluster of Isohypsibius granulifer granulifer is branched. The cytoplasmic briges possess two rims: an electron-dense outer rim and an inner rim of a medium electron density. The trophocytes participate in the yolk synthesis. Autophagy and apoptosis appear in the trophocytes during late choriogenesis in I. g. granulifer . Trophocytes die in an apoptotic way. Abstract: The studies on the fates of the trophocytes, the apoptosis and autophagy in the gonad of Isohypsibius granulifer granulifer have been described using transmission electron microscope, light and fluorescent microscopes. The results presented here are the first that are connected with the cell death of nurse cells in the gonad of tardigrades. However, here we complete the results presented byWęglarska (1987) . The reproductive system of I. g. granulifer contains a single sack-like hermaphroditic gonad and a single gonoduct. The gonad is composed of three parts: a germarium filled with proliferating germ cells (oogonia); a vitellarium that has clusters of female germ cells (the region of oocytes development); and a male part filled with male germ cells in which the sperm cells develop. The trophocytes (nurse cells) show distinct alterations during all of the stages of oogenesis: previtello-, vitello- and choriogenesis. During previtellogenesis the female germ cells situated in the vitellarium are connected by cytoplasmic bridges, and form clusters of cells. No ultrastructural differences appear among the germ cells in a cluster during this stage of oogenesis. In early vitellogenesis, the cells in each cluster start to grow and numerous organelles gradually accumulate in their cytoplasm. However, at the beginning of the middle of vitellogenesis, one cell in each cluster starts to grow in order to differentiate into oocyte, while the remaining cells are trophocytes. Eventually, the cytoplasmic bridges between the oocyte and trophocytes disappear. Autophagosomes also appear in the cytoplasm of nurse cells together with many degenerating organelles. The cytoplasm starts to shrink, which causes the degeneration of the cytoplasmic bridges between trophocytes. Apoptosis begins when the cytoplasm of these cells is full of autophagosomes/autolysosomes and causes their death.