Mature adipocyte proteome reveals differentially altered protein abundances between lean, overweight and morbidly obese human subjects. (5th February 2015)

• Record Type: Journal Article
• Authors: Benabdelkamel, Hicham; Masood, Afshan; Almidani, Ghaith M.; Alsadhan, Abdulmajeed A.; Bassas, Abdulelah F.; Duncan, Mark W.; Alfadda, Assim A.
• Is Part Of: Molecular and cellular endocrinology. Volume 401(2015)
• Page Start: 142
• Page End: 154
• Publication Date: 2015-02-05
• Subjects: AFABP adipocyte fatty acid-binding protein -- APOA-I apolipoprotein A-I -- ATP adenosine triphosphate -- BMI body mass index -- CVD cardiovascular disease -- 2D-DIGE 2 dimensional difference in-gel electrophoresis -- ECM extracellular matrix -- ETC electron transport chain -- ECHM enoyl CoA hydratase mitochondrial -- EFABP epidermal fatty acid binding protein -- GLUT 4 glucose transporter 4 -- Hb hemoglobin -- IEF isoelectric focusing -- IPA Ingenuity Pathway Analysis -- LDL low-density lipoprotein -- L lean -- MALDI matrix-assisted laser desorption/ionization -- MS mass spectrometry -- MOB morbidly obese -- OW overweight -- SCAT subcutaneous adipose tissue -- SD standard deviation -- SCAD short chain acyl-coenzyme A dehydrogenase -- TCA tricarboxylic citric acid cycle -- TOF time-of-flight -- VAT visceral adipose tissue -- WAT white adipose tissue; Obesity -- Lean -- Overweight -- Obese -- Human adipocytes -- Proteomics; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2014.11.021
• Languages: English
• ISSNs: 0303-7207

Abstract

Highlights: Mature adipocytes differ in metabolisms in the overweight andmorbid obese . 2D-DIGE and MALDI-TOF/TOF identified differences in protein abundance between overweight and morbid obese compared to lean. Network pathway identified different pathways active in the overweight and morbid obese. The proteins identified in this study might contribute to understanding the metabolism with weight gain. Abstract: Overweight (OW) and obese individuals are considered to be graded parts of the scale having increasing weight as a common feature. They may not, however, be part of the same continuum and may differ metabolically. In this study we applied an untargeted proteomic approach to compare protein abundances in mature adipocytes derived from the subcutaneous adipose tissue of overweight and morbidly obese female subjects to those of lean age matched controls. Mature adipocytes were isolated from liposuction samples of abdominal subcutaneous adipose tissue collected from both lean (L; n = 7, 23.3 ± 0.4 kg/m 2 ; mean BMI ± SD), overweight (OW; n = 8, 27.9 ± 0.6 kg/m 2 ; mean BMI ± SD) and morbidly obese (MOB; n = 7, 44.8 ± 3.8 kg/m 2 ; mean BMI ± SD) individuals. Total protein extracts were then compared by two-dimensional difference in gel electrophoresis (2D DIGE). One hundred and ten differentially expressed protein spots (i.e., fitting the statistical criteria ANOVA test, p < 0.05; fold-change ≥1.5) were detected, and of these, 89 were identified by MALDI-TOF mass spectrometry. Of these, 66 protein spots were common to both groups whereas 23 were unique to the MOB group. Significant differences were evident in the abundances of key proteins involved in glucose and lipid metabolism, energy regulation, cytoskeletal structure and redox control signaling pathways. Differences in the abundance of some chaperones were also evident. The differentially abundant proteins were investigated using Ingenuity Pathway Analysis (IPA) to establish their associations with known biological functions. The network identified in the OW group with the highest score relates to-: cell-to-cell signaling and interaction; in contrast, in the MOB group the major interacting pathways are associated with lipid metabolism, small molecule biochemistry and cancer. The differences in abundance of the differentially regulated proteins were validated by immunoblotting. These findings provide insights into metabolic differences in OW and MOB individuals.