Cilostazol strengthens the endothelial barrier of postcapillary venules from the rat mesentery in situ. (October 2014)

• Record Type: Journal Article
• Authors: Sugiura, Yasoo; Morikawa, Takayuki; Takenouchi, Toshiki; Suematsu, Makoto; Kajimura, Mayumi
• Is Part Of: Phlebology. Volume 29:Number 9(2014)
• Page Start: 594
• Page End: 599
• Publication Date: 2014-10
• Subjects: Microvascular permeability -- antiplatelet drugs -- phosphodiesterase 3; Veins -- Diseases -- Periodicals; 616.14
• DOI: 10.1177/0268355513497361
• Languages: English
• ISSNs: 0268-3555

Abstract

Objective: Although cilostazol, a phosphodiesterase 3 inhibitor, has been suggested to strengthen the endothelial barrier using cultured endothelial monolayers, its effect has not been tested in vivo . We, therefore, investigated effects of cilostazol on barrier properties of postcapillary venules of the rat in situ . Methods: Cilostazol was administered to the rats through oral gavage at 4 hours before the measurements. The hydraulic permeability ( L p ) and the effective osmotic pressure (σΔπ), molecular sieving properties of microvascular walls, were estimated in single mesenteric postcapillary venules by a micro-occlusion technique, first during control perfusion and then in the presence of histamine. Results: When the vessels were inflamed with histamine, cilostazol attenuated a transient increase in L p and prevented σΔπ from falling. Furthermore, it reduced baseline L p under a control state. Conclusion: Cilostazol appears to tighten the endothelial barrier in situ, at least in part by inhibiting the cAMP-degrading enzyme in the endothelium.