Metabolomic profiling in multiple sclerosis: insights into biomarkers and pathogenesis. (September 2014)

• Record Type: Journal Article
• Authors: Reinke, SN; Broadhurst, DI; Sykes, BD; Baker, GB; Catz, I; Warren, KG; Power, C
• Is Part Of: Multiple sclerosis. Volume 20:Number 10(2014)
• Page Start: 1396
• Page End: 1400
• Publication Date: 2014-09
• Subjects: Multiple sclerosis -- metabolomics -- biomarker -- nuclear magnetic resonance -- cerebrospinal fluid; Central nervous system -- Diseases -- Periodicals; Myelin sheath -- Diseases -- Periodicals; Inflammation -- Periodicals; Multiple sclerosis -- Periodicals; Central Nervous System Diseases -- Periodicals; Demyelinating Diseases -- Periodicals; Inflammation -- Periodicals; Multiple Sclerosis -- Periodicals; Système nerveux central -- Maladies -- Périodiques; Gaine de myéline -- Maladies -- Périodiques; Inflammation (Pathologie) -- Périodiques; Sclérose en plaques -- Périodiques; Electronic journals; 616.834005
• DOI: 10.1177/1352458513516528
• Languages: English
• ISSNs: 1352-4585

Abstract

Metabolomics enables the provision of sensitive bio-markers of disease. We performed 800 MHz 1 H-nuclear magnetic resonance (NMR) spectroscopic analyses of cerebrospinal fluid (CSF) specimens to identify biomarkers of multiple sclerosis (MS), yielding reproducible detection of 15 metabolites from MS ( n =15) and non-MS ( n =17) patients. Mean levels of choline, myo-inositol and threonate were increased, whereas 3-hydroxybutyrate, citrate, phenylalanine, 2-hydroxyisovalerate and mannose were decreased in MS-derived CSF ( p <0.05), suggesting alterations to energy and phospholipid metabolism. Multivariate hierarchal cluster analysis indicated a high correlation within the metabolite profiles, significantly clustering samples into the two clinical groups, which was corroborated using principal components analysis. CSF metabolomics have the capacity to yield quantitative biomarkers and insights into the pathogenesis of MS.