Localization of sirtuins (SIRT1-7) in the aged mouse inner ear. (1st February 2016)

• Record Type: Journal Article
• Authors: Takumida, Masaya; Takumida, Hiroshi; Katagiri, Yoshiaki; Anniko, Matti
• Is Part Of: Acta oto-laryngologica. Volume 136:Number 2(2016)
• Page Start: 120
• Page End: 131
• Publication Date: 2016-02-01
• Subjects: Sirtuin -- inner ear -- mouse -- ageing -- presbycusis; Otolaryngology -- Periodicals; Ear -- Diseases -- Periodicals; Throat -- Diseases -- Periodicals; Otolaryngology -- Electronic Resources; Otorhinolaryngologic Diseases; 617.8
• DOI: 10.3109/00016489.2015.1093172
• Languages: English
• ISSNs: 0001-6489

Abstract

Abstract: Conclusion : The expression of sirtuin in vestibular end organs and cochlea responds differently to age-related changes. Down-regulation of SIRT1, 3, and 5 in the cochlea may weaken the protective activity regarding degeneration of the organ of Corti as well as of spiral ganglion cells, resulting in the development of age-related hearing loss. An increase in SIRT 1, 4, or 5 in vestibular tissue could indicate an increased need of detoxification of reactive oxygen species and an increased anti-ageing potential. Objective : To analyse the expression of sirtuins (SIRT1-7) in the normal young and old mouse inner ears. Methods : Young (8 weeks) and old (22 months) CBA/J mice were used in this study. Localization of SIRT1-7 in the inner ear, i.e. cochlea, vestibular end organs, and vestibular ganglion, was investigated using real-time PCR and immunohistochemistry. Results : In the vestibular end organs, the expression of SIRT1, 2, 4, 5 (both mRNA and protein), SIRT6, and 7 (only mRNA) was found to be increased, while a slightly decreased immunoreactivity was observed in SIRT3. In the cochlea, the expression of SIRT1, 3, and 5 (both mRNA and protein) was decreased in the old mice, whereas no noticeable difference was observed regarding SIRT2, 4, 6, or 7.