Cell wall damage and oxidative stress in Candida albicans ATCC10231 and Aspergillus niger caused by palladium nanoparticles. (April 2018)

• Record Type: Journal Article
• Authors: Athie-García, Martha Samira; Piñón-Castillo, Hilda Amelia; Muñoz-Castellanos, Laila Nayzzel; Ulloa-Ogaz, Ana Laura; Martínez-Varela, Perla Ivonne; Quintero-Ramos, Armando; Duran, Robert; Murillo-Ramirez, José Guadalupe; Orrantia-Borunda, Erasmo
• Is Part Of: Toxicology in vitro. Volume 48(2018)
• Page Start: 111
• Page End: 120
• Publication Date: 2018-04
• Subjects: Microscopy -- Toxicity -- Reactive oxygen species -- Intracellular integrity loss; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2018.01.006
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: In this work the toxic effect of Palladium nanoparticles (PdNPs) was investigated in two eukaryotic cell models, Candida albicans and Aspergillus niger . PdNPs were synthesized by chemical reduction method, obtaining spherical NPs with a primary size ranging from 3 to 15 nm. PdNPs showed a hydrodynamic size of 1548 nm in Lee's minimum media. Minimal inhibitory concentration was determined at 200 and 250 ppm for Candida albicans and Aspergillus niger respectively, revealing a significant cell growth inhibition (ANOVA and tukey analysis, α = 0.5). Reactive Oxygen Species levels were increased in both microorganisms. Confocal, scanning and transmission electron microscopy studies revealed cell wall damage and cellular morphology changes, induced by the interaction of PdNPs, in both microorganisms. Highlights: PdNPs toxic effects in Candida albicans and Aspergillus niger was studied. The PdNPs toxic effects were mediated by cell wall damage and oxidative stress. The antifungal activity depended of evaluated fungi. Loss in the integrity of intracellular structures was evident.