Pterocarpus santalinus L. Regulated Ultraviolet B Irradiation‐induced Procollagen Reduction and Matrix Metalloproteinases Expression Through Activation of TGF‐β/Smad and Inhibition of the MAPK/AP‐1 Pathway in Normal Human Dermal Fibroblasts. (3rd November 2017)

• Record Type: Journal Article
• Authors: Gao, Wei; Lin, Pei; Hwang, Eunson; Wang, Yushuai; Yan, Zhengfei; Ngo, Hien T.T.; Yi, Tae‐Hoo
• Is Part Of: Photochemistry and photobiology. Volume 94:Number 1(2018)
• Page Start: 139
• Page End: 149
• Publication Date: 2017-11-03
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.12835
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: Ultraviolet light‐induced reactive oxygen species (ROS) damage human skin and prematurely cause aging. A growing body of research is focusing on considering plants and plant‐derived compounds as antiphotoaging therapeutic material. Pterocarpus santalinus L., as an Indian traditional medicine, possesses antidiabetic, anti‐inflammatory and antioxidative effects. Here, we studied the antiphotoaging effects of ethanolic extract of P. santalinus L. heartwood (EPS) on ultraviolet radiation B (UVB)‐irradiated normal human dermal fibroblasts (NHDFs). Results showed that EPS significantly inhibited the upregulation of matrix metalloproteinases and IL‐6 caused by UVB irradiation, and suppressed UVB‐induced phosphorylation of extracellular signal‐regulated kinase, Jun N‐terminal kinase and p38, as well as the activation of AP‐1 transcription factors. Further study indicated that UVB‐induced production of MMP‐1 and IL‐6 could be inhibited by PD 98059 (an ERK inhibitor) and SP600125 (A JNK inhibitor), implied that EPS inhibited UVB‐induced MMP‐1 and IL‐6 secretion by inactivating MAPK signaling pathway. In addition, EPS possessed an excellent antioxidant activity, which could increase cytoprotective antioxidants such as HO‐1, NQ‐O1 expression by facilitating the nuclear accumulation of Nrf2. Treatment of NHDFs with EPS also recovered UVB‐induced procollagen type I reduction by activating TGF‐ β /Smad pathway. These findings demonstrated that EPS had a potential effect against UVB‐induced skin photoaging. Abstract : Chronic exposure to UV radiation results in oxidative damage to skin, reflecting a clinically photoaging with loss of elasticity and tension. In this study, we demonstrated that EPS could be served as a potential photoprotective agent against UVB‐induced skin aging. The protection mechanism involved in the increase of type‐I procollagen synthesis and blocking of MMPs expression which was referred to the regulation of TGF‐ β /Smad and MAPK/AP‐1 pathway. Furthermore, EPS could decrease the ROS level by accelerating the Nrf2 nuclear location and enhancing the expression of cytoprotective antioxidant.