Biocatalytic Synthesis of Phospholipids and Their Application as Coating Agents for CaCO3 Nano–crystals: Characterization and Intracellular Localization Analysis. Issue 20 (15th December 2016)

• Record Type: Journal Article
• Authors: Baldassarre, Francesca; Allegretti, Chiara; Tessaro, Davide; Carata, Elisabetta; Citti, Cinzia; Vergaro, Viviana; Nobile, Concetta; Cannazza, Giuseppe; D'Arrigo, Paola; Mele, Andrea; Dini, Luciana; Ciccarella, Giuseppe
• Is Part Of: ChemistrySelect. Volume 1:Issue 20(2016)
• Page Start: 6507
• Page End: 6514
• Publication Date: 2016-12-15
• Subjects: Biocatalytic synthesis -- CaCO3 nano-crystals -- Drug Delivery Systems -- Phospholipids -- Plasmatic Membrane; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201601429
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Inorganic nanoparticles are widely investigated as drug delivery systems. In particular micro and nano‐particles of CaCO3 offer smart features for different biomaterials applications. In this work we exploit the phospholipids coating of nano‐CaCO3 . We prepare modified phospholipids through a chemo‐enzymatic approach using Phospholipase D to efficiently transform the most abundant natural phospholipids in two products, sn‐glycero‐3‐phosphocholine (GPC) and sn‐glycero‐3‐phosphoserine (GPS). We have investigated and modified the Spray Drier process to obtain nano‐crystals with specific phase, surface ζ‐potential and morphology. The intracellular localization of coated nano‐crystals was achieved by ultrastructural microscopy analysis. The synthetized phospholipids, GPC and GPS, improve nano‐CaCO3 proprieties and promote a specific targeting as opposed to a widespread and nonspecific localization in the cell. Abstract : Smart targeted delivery systems: We investigated a biocatalytic synthesis to obtain two Phospholipids. These molecules are exploited as coating agents for CaCO3 nanocrystals which are obtained through a Spray Drier process. This coating resulted to tune the colloidal and biological behavior of nano‐CaCO3 . The Plasmatic Membrane targeting could be provided.