Sources of paralytic shellfish toxin accumulation variability in the Pacific oyster Crassostrea gigas. (15th March 2018)

• Record Type: Journal Article
• Authors: Pousse, Émilien; Flye-Sainte-Marie, Jonathan; Alunno-Bruscia, Marianne; Hégaret, Hélène; Jean, Fred
• Is Part Of: Toxicon. Volume 144(2018)
• Page Start: 14
• Page End: 22
• Publication Date: 2018-03-15
• Subjects: Alexandrium minutum -- Paralytic shellfish poisoning -- Accumulation -- Clearance rate -- Feeding behavior -- Pacific oyster; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2017.12.050
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: This study was designed to assess the contribution of feeding behavior to inter-individual variability of paralytic shellfish toxin (PST) accumulation in the Pacific oyster Crassostrea gigas . For this purpose 42 oysters were exposed for 2 days to non-toxic algae and then for 2 other days to the PST producer Alexandrium minutum . Individual clearance rate (CR) of oysters was continuously monitored over the 4 days using an ecophysiological measurement system. Comparison of CR values when exposed to toxic and non toxic algae allowed to estimate a clearance rate inhibition index (CRII). Toxin concentration of oysters was quantified at the end of the experiment. These data allowed to estimate the toxin accumulation efficiency (TAE) as the ratio of toxin accumulated on toxin consumed. Changes of clearance rate during the experiment indicated that all individuals stopped feeding immediately after being exposed to A. minutum for at least 7 h. This fast response likely corresponded to a behavioral mechanism of avoidance rather to a toxin-induced response. Individuals also showed high inter-variability in their recovery of filtration after this period. Most of the inter-individual variability (78%) in PST accumulation in C. gigas could be explained by the consumption of A. minutum cells, thus emphasizing the importance of the feeding behavior in accumulation. Based on the toxin concentration in their tissues, oysters were clustered in 3 groups showing contrasted patterns of PST accumulation: the high accumulation group was characterized by high feeding rates both on non-toxic and toxic diet and subsequently a low CRII and high TAE. Inversely, the low accumulation group was characterized by low filtration rates, high CRII and low TAE. Both filtration capacity and sensitivity of oysters to toxins may account for the differences in their accumulation. The contribution of TAE in PST accumulation is discussed and might result from differences in assimilation and detoxification abilities among individuals. Highlights: Facing exposure to A. minutum, individual clearance rates of oysters were measured. Algal consumption explains variability in paralytic shellfish toxin accumulation. Three phenotypes were identified on the basis of their accumulation potential. Phenotypes differed in their feeding rates. Phenotypes also differed in their sensitivity to toxins and accumulation efficiency.