Oxidative imbalance in mice intoxicated by microcystin-LR can be minimized. (15th March 2018)

• Record Type: Journal Article
• Authors: Casquilho, Natália V.; Moreira-Gomes, Maria Diana; Magalhães, Clarissa B.; Okuro, Renata T.; Ortenzi, Victor Hugo; Feitosa-Lima, Emanuel K.; Lima, Lidia M.; Barreiro, Eliezer J.; Soares, Raquel M.; Azevedo, Sandra M.F.O.; Valença, Samuel S.; Fortunato, Rodrigo S.; Carvalho, Alysson Roncally; Zin, Walter A.
• Is Part Of: Toxicon. Volume 144(2018)
• Page Start: 75
• Page End: 82
• Publication Date: 2018-03-15
• Subjects: cyanobacteria -- Oxidative stress -- LASSBio-596 -- Liver injury -- Lung mechanics -- Microcystin-LR; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2018.02.008
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Microcystins-LR (MC-LR) is a cyanotoxin produced by cyanobacteria. We evaluated the antioxidant potential of LASSBio-596 (LB-596, inhibitor of phosphodiesterases 4 and 5), per os, and biochemical markers involved in lung and liver injury induced by exposure to sublethal dose of MC-LR. Fifty male Swiss mice received an intraperitoneal injection of 60 μL of saline (CTRL group, n = 20) or a sublethal dose of MC-LR (40 μg/kg, TOX group, n = 20). After 6 h the animals received either saline (TOX and CTRL groups) or LB-596 (50 mg/kg, TOX + LASS group, n = 10) by gavage. At 6 h after exposure, respiratory mechanics was evaluated in 10 CTRL and 10 TOX mice: there was a significant increase of all lung mechanics parameters (static elastance, viscoelastic component of elastance and lung resistive and viscoelastic/inhomogeneous pressures) in TOX compared to CTRL. 8 h after saline or MC-LR administration, i.e., 2 h after treatment with LB-596, blood serum levels of alanine aminotransferase and aspartate aminotransferase, activity of superoxide dismutase, catalase, and content of malondialdehyde and carbonyl in lung and liver, NADPH oxidase 2 and 4 mRNA expressions, dual oxidase enzyme activity and H2 O2 generation were analyzed in lung homogenates. All parameters were significantly higher in TOX than in the other groups. There was no significant difference between CTRL and TOX + LASS. MC-LR deteriorated lung and liver functions and induced redox imbalance in them, which was prevented by oral administration of LB-596. Highlights: The effects of LASSBio 596 (LB-596) per os were evaluated in an acute model of intoxication by microcystin-LR (MC-LR). MC-LR impaired lung and liver functions, as well as produced oxidative stress and damage. Treatment with LB-596 improved lung and liver oxidative stress and damage and recovered liver function.