A supplement‐free osteoclast–osteoblast co‐culture for pre‐clinical application. Issue 6 (23rd August 2017)

• Record Type: Journal Article
• Authors: Schulze, Sabine; Wehrum, Diana; Dieter, Peter; Hempel, Ute
• Is Part Of: Journal of cellular physiology. Volume 233:Issue 6(2018:Jun.)
• Page Start: 4391
• Page End: 4400
• Publication Date: 2017-08-23
• Subjects: bisphosphonates -- bone marrow stromal cells -- co‐culture -- glycosaminoglycans -- osteoblasts -- osteoclasts -- peripheral blood mononuclear cells; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.26076
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : There is increasing demand for efficient and physiological in vitro cell culture systems suitable for testing new pharmaceutical drugs or for evaluating materials for tissue regeneration. In particular, co‐cultures of two or more tissue‐relevant cell types have the advantage to study the response of cells on diverse parameters in a more natural environment with respect to physiological complexity. We developed a direct bone cell co‐culture system using human peripheral blood monocytes (hPBMC) and human bone marrow stromal cells (hBMSC) as osteoclast/osteoblast precursor cells, respectively, strictly avoiding external supplements for the induction of differentiation. The sophisticated direct hPBMC/hBMSC co‐culture was characterized focusing on osteoclast function and was compared with two indirect approaches. Only in the direct co‐culture, hPBMC were triggered by hBMSC into osteoclastogenesis and became active resorbing osteoclasts. Bisphosphonates and sulfated glycosaminoglycans were used to examine the suitability of the co‐culture system for evaluating the influence of certain effectors on bone healing and bone regeneration and the contribution of each cell type thereby. The results show that the investigated substances had more pronounced effects on both osteoblasts and osteoclasts in the co‐culture system than in respective monocultures. Abstract : In a supplement‐free direct co‐culture, hPBMC and hBMSC differentiated into osteoclasts and osteoblasts. On these co‐cultured cells, bisphosphonates and glycosaminoglycans had more pronounced effects than in respective monocultures.