Development of a LC–MS/MS method for the determination of isomeric glutamyl peptides in food ingredients. Issue 4 (18th December 2017)

• Record Type: Journal Article
• Authors: Desfontaine, Vincent; Goyon, Alexandre; Veuthey, Jean‐Luc; Charve, Joséphine; Guillarme, Davy
• Is Part Of: Journal of separation science. Volume 41:Issue 4(2018)
• Page Start: 847
• Page End: 855
• Publication Date: 2017-12-18
• Subjects: amide stationary phases -- food ingredients -- glutamyl peptides -- hydrophilic interaction chromatography -- structural isomers; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201701182
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: A liquid chromatography with tandem mass spectrometry method was developed for the determination of 27 glutamyl di‐ and tripeptides in food ingredients. Such compounds are of importance for the food industry, as they can modulate the perception of basic tastes (sweet, salty, and umami). Due to their high polarity, the hydrophilic interaction chromatography mode was selected to have sufficient retention on the column and the best separation was obtained on an amide hybrid silica stationary phase packed with 1.7 μm particles. Thorough optimization of the mobile phase was performed as the start‐composition had to be free of ammonium to avoid on‐column cis – trans isomerization of the first eluting proline dipeptide. A baseline separation was achieved for all α and γ isomers whereas only a partial resolution was obtained for γ‐Glu‐Leu and γ‐Glu‐Ile, for which only the position of a methyl group differs. A fast sample preparation, based on successive dilutions, was performed before injection into the liquid chromatography with tandem mass spectrometry system. The developed method was then applied for the semi‐quantification of glutamyl di‐ and tri‐peptides in four different food ingredients. The methodology will further support the optimization of production processes to select the conditions for which the peptide concentrations would be the highest.