Post-translational maturation of IDA, a peptide signal controlling floral organ abscission in Arabidopsis. Issue 1 (2nd January 2018)

• Record Type: Journal Article
• Authors: Stührwohldt, Nils; Hohl, Mathias; Schardon, Katharina; Stintzi, Annick; Schaller, Andreas
• Is Part Of: Communicative & integrative biology. Volume 11:Issue 1(2018)
• Publication Date: 2018-01-02
• Subjects: Abscission -- Arabidopsis thaliana -- IDA -- post-translational modification -- precursor maturation -- proteolytic processing -- peptide signaling -- subtilase -- proline hydroxylation; Biology -- Periodicals; Molecular biology -- Periodicals; 579.05
• DOI: 10.1080/19420889.2017.1395119
• Languages: English
• ISSNs: 1942-0889

Abstract

ABSTRACT: The abscission of sepals, petals and stamens in Arabidopsis flowers is controlled by a peptide signal called IDA (Inflorescence Deficient in Abscission). IDA belongs to the large group of small post-translationally modified signaling peptides that are synthesized as larger precursors and require proteolytic processing and specific side chain modifications for signal biogenesis. Using tissue-specific expression of proteinase inhibitors as a novel approach for loss-of-function analysis, we recently identified the peptidases responsible for IDA maturation within the large family of subtilisin-like proteinases (subtilases; SBTs). Further biochemical and physiological assays identified three SBTs (AtSBT5.2, AtSBT4.12, AtSBT4.13) that cleave the IDA precursor to generate the N-terminus of the mature peptide. The C-terminal processing enzyme(s) remain(s) to be identified. While proline hydroxylation was suggested as additional post-translational modification required for IDA maturation, hydroxylated and non-hydroxylated IDA peptides were found to be equally active in bioassays for abscission.