Evaluation of the modified carbapenem inactivation method for the detection of carbapenemase-producing Enterobacteriaceae. Issue 4 (April 2018)

• Record Type: Journal Article
• Authors: Kuchibiro, Tomokazu; Komatsu, Masaru; Yamasaki, Katsutoshi; Nakamura, Tatsuya; Nishio, Hisaaki; Nishi, Isao; Kimura, Keigo; Niki, Makoto; Ono, Tamotsu; Sueyoshi, Noriyuki; Kita, Machiko; Kida, Kaneyuki; Ohama, Masanobu; Satoh, Kaori; Toda, Hirofumi; Mizutani, Tetsu; Fukuda, Nozomi; Sawa, Kana; Nakai, Isako; Kofuku, Tomomi; Orita, Tamaki; Watari, Hideo; Shimura, Satoshi; Fukuda, Saori; Nakamura, Akihiro; Wada, Yasunao
• Is Part Of: Journal of infection and chemotherapy. Volume 24:Issue 4(2018:Apr.)
• Page Start: 262
• Page End: 266
• Publication Date: 2018-04
• Subjects: Modified carbapenem inactivation method test -- Modified Hodge test -- Carba NP test -- Carbapenemase-producing Enterobacteriaceae; CIM carbapenem inactivation method -- CNP Carba NP test -- CPE carbapenemase-producing Enterobacteriaceae -- ESBLs extended-spectrum β-lactamases -- IPM imipenem -- mCIM modified carbapenem inactivation method -- MEPM meropenem -- MHA Mueller Hinton agar -- MHT modified Hodge test -- MICs minimum inhibitory concentrations -- TSB trypticase soy broth; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2017.11.010
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: Carbapenemase-producing Enterobacteriaceae (CPE) are increasing worldwide. Rapid and accurate detection of CPE is necessary for appropriate antimicrobial treatment and hospital infection control. However, CPE contains some strains that are difficult to detect depending on genotype and MIC value of carbapenem, and a detection method has not been established. The recently reported modified carbapenem inactivation method (mCIM) has been developed in CLSI M100-S27 as a phenotypic technique for detecting carbapenemase activity. In the present study, we examined mCIM as a new CPE detection method using 207 Enterobacteriaceae isolates in comparison with the three existing screening methods of modified Hodge test, Carba NP test and carbapenem inactivation method and evaluated its performance. Consequently, both the sensitivity and specificity of mCIM were 100%, indicating better results than the conventional screening methods. The mCIM is a useful tool for microbiology laboratories due to its simplicity, clear criteria, cost-effectiveness and availability at any laboratory.