Regulation of protein‐binding activities of molecularly imprinted polymers via post‐imprinting modifications to exchange functional groups within the imprinted cavity. Issue 3 (11th April 2017)

• Record Type: Journal Article
• Authors: Sunayama, Hirobumi; Kitayama, Yukiya; Takeuchi, Toshifumi
• Other Names: Spivak David A. guestEditor.
• Is Part Of: Journal of molecular recognition. Volume 31:Issue 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-04-11
• Subjects: molecular imprinting -- polymers -- post‐imprinting modification -- sensor -- synthetic receptor; Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2633
• Languages: English
• ISSNs: 0952-3499

Abstract

Abstract: We prepared lysozyme‐imprinted polymers bearing modifiable sites within the imprinted cavity to introduce various functional groups via post‐imprinting modifications. For this purpose, ({[2‐(2‐methacrylamido)‐ethyldithio]‐ethylcarbamoyl}‐methoxy)acetic acid (MDTA), which has a carboxy group to interact with the target protein, lysozyme, and a disulfide linkage for post‐imprinting modifications, was used as a functional monomer. A lysozyme‐imprinted polymer film was prepared by copolymerization of MDTA with a cross‐linker, N, N ′‐methylenebisacrylamide, in the presence of lysozyme. After removing lysozyme, followed by reducing the disulfide linkage, various functional groups, such as carboxy, amino, sulfonate, and oligo‐ethylene oxide, were introduced to the exposed thiol groups via a disulfide exchange reaction using the pyridyldisulfide derivatives of these functional groups. Various functional groups could be introduced reversibly via this post‐imprinting disulfide exchange reaction after the construction of the lysozyme‐imprinted cavities. The modification regulated the protein‐binding activity. The proposed post‐imprinting modification system, based on a molecular imprinting process, is expected to lead to the development of advanced materials for fine‐tuning and/or introducing desired functions. Abstract : Protein‐imprinted cavity with modifiable moieties was prepared and reversibly introduced various functional groups via post‐imprinting modifications. Protein‐binding activity was regulated successfully by exchanging these functional groups as post‐imprinting modifications.