Laterally and Temporally Controlled Intracellular Staining by Light‐Triggered Release of Encapsulated Fluorescent Markers. Issue 9 (17th January 2018)

• Record Type: Journal Article
• Authors: Kantner, Karsten; Rejman, Joanna; Kraft, Karl V. L.; Soliman, Mahmoud G.; Zyuzin, Mikhail V.; Escudero, Alberto; del Pino, Pablo; Parak, Wolfgang J.
• Is Part Of: Chemistry. Volume 24:Issue 9(2018)
• Page Start: 2098
• Page End: 2102
• Publication Date: 2018-01-17
• Subjects: capsules -- fluorescent probes -- immunostaining -- single cells; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201706135
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Fluorescent molecular markers were encapsulated. The capsules were additionally modified with plasmonic nanoparticles. The encapsulated markers were endocytosed by cells. Upon light stimulation the plasmonic nanoparticles generated heat, which opened the encapsulation and transiently perforated the endosomal/lysosomal membrane surrounding the capsule, thus allowing for release of the marker into the cytosol. Fluorescence labeling of different intracellular compartments was demonstrated in this way. Most important, the cells do not need to be fixed and perforated, as the molecular markers are introduced into cells by endocytosis and subsequent light‐induced release. Thus this technique allows for intracellular fluorescence labeling of living cells. Abstract : Lighting up single cells : Encapsulated molecular markers can be intracellularly released upon an external light trigger and stain intracellular structures in living cells (see figure). Importantly, the cells do not need to be fixed and perforated, as the molecular markers are introduced into cells by endocytosis.