Folding DNA into a Lipid‐Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins. (9th January 2018)

• Record Type: Journal Article
• Authors: Dong, Yuanchen; Chen, Shuobing; Zhang, Shijian; Sodroski, Joseph; Yang, Zhongqiang; Liu, Dongsheng; Mao, Youdong
• Is Part Of: Angewandte Chemie. Volume 130:Number 8(2018)
• Page Start: 2094
• Page End: 2098
• Publication Date: 2018-01-09
• Subjects: DNA-Nanotechnologie -- DNA-Origami -- Einzelpartikelanalyse -- Kryo-Elektronenmikroskopie -- Membranprotein-Rekonstitution; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201710147
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self‐assembled DNA nanobarrel that scaffolds a nanodisc‐like lipid environment. Compared with the membrane‐scaffolding‐protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins.