A Rapid Method for Determining the Oxidative Stability of Oils Suitable for Breeder Size Samples. (21st April 2013)

• Record Type: Journal Article
• Authors: Przybylski, R.; Wu, J.; Eskin, N. A. M.
• Is Part Of: Journal of the American Oil Chemists' Society. Volume 90:Number 7(2013)
• Page Start: 933
• Page End: 939
• Publication Date: 2013-04-21
• Subjects: TLC‐FID -- Polar material -- Oxidative stability -- Oxidation; Oils and fats -- Periodicals; Soap -- Periodicals; Fats -- Periodicals; Food-Processing Industry -- Periodicals; Acides gras -- Périodiques; Aliments -- Industrie et commerce -- Périodiques; Huiles et graisses -- Périodiques; Savon -- Périodiques; Oliën; Chemie; Oils and fats; Soap; Periodicals; 547.77
• DOI: 10.1007/s11746-013-2240-1
• Languages: English
• ISSNs: 0003-021X

Abstract

Abstract: A method utilizing thin‐layer chromatography with a flame ionization detector (TLC‐FID) was developed for assessing the stability of breeder's oil seed samples based on the formation of polar compounds. The results showed a linear relationship between peroxide value (PV) and the content of polar material in the oxidized oil. Oil samples oxidized very readily on chromarods, even at low temperature, which is a particular advantage for antioxidant screening. At 45 °C, the oil oxidation rate was relatively low, but the relationship between the content of polar material and reaction time was linear. At 65 °C, if the content of polar material was below 50 %, the above relationship was still linear. At different temperatures, the action of tocopherol appeared to vary slightly. For example, at 65 °C, the oxidative stability of the oil sample was determined by the content of tocopherol, especially γ‐tocopherol. At 45 and 55 °C, the oxidative stability was determined by both the content of tocopherol and polyunsaturated fatty acids. Of the tocopherol isomers, γ‐tocopherol exhibited the highest antioxidant potency, consistent with the published literature. These results suggest that chromarods provide good media for monitoring oil oxidation for antioxidant screening. A particular advantage is the use of very small oil samples, usually 1–2 μL, and the ability to analyze multiple samples at the same time.