Demodex canis targets TLRs to evade host immunity and induce canine demodicosis. (7th January 2018)

• Record Type: Journal Article
• Authors: Kumari, P.; Nigam, R.; Choudhury, S.; Singh, S. K.; Yadav, B.; Kumar, D.; Garg, S. K.
• Is Part Of: Parasite immunology. Volume 40:Number 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-01-07
• Subjects: generalized demodicosis -- innate immune response -- localized demodicosis -- TLR2 -- TLR4 -- TLR6; Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12509
• Languages: English
• ISSNs: 0141-9838

Abstract

Summary: Widespread incidence of Demodex mites throughout the mammalian class and occasional serious and fatal outcomes in dogs warrant an insight into the host‐parasite interface especially. Therefore, this study was aimed to unravel the interplay between innate immune response and canine demodicosis. The dogs diagnosed to have natural clinical demodicosis were allocated into two groups; dogs with localized demodicosis (LD) and with generalized demodicosis (GD). The expression of toll‐like receptors (TLRs) 2, 4 and 6 genes in peripheral blood mononuclear cells of these dogs was quantified by real‐time PCR. Significantly increased TLR2 gene expression, while significantly diminished TLR4 and TLR6 gene expressions were observed in demodicosed dogs (LD and GD) as compared with the healthy ones. Even the expression of TLR2 gene was found to differ significantly between the dogs with LD and GD. Therefore, it can be inferred that clinical demodicosis in dogs is coupled with an up‐regulation of TLR2 and down‐regulation of TLR4 and TLR6 gene expressions. Overexpression of TLR2 gene might be responsible for Demodex‐ induced clinical manifestations, while TLR4 and TLR6 gene down‐regulations could be the paramount strategy of Demodex mites to elude the host‐immune interface.