Really does temperature reduction and norepinephrine have similar effects on the energy metabolism in rat brown adipose tissue?. (1st January 2018)

• Record Type: Journal Article
• Authors: Sopeña, B.; López-Ibarra, Z.; López-Farré, A. J.; de las Heras, N.; Ballesteros, S.; González-Cantalapiedra, A.; Lahera, V.; Zamorano-León, J. J.
• Is Part Of: Archives of physiology and biochemistry. Volume 124:Number 1(2018)
• Page Start: 54
• Page End: 60
• Publication Date: 2018-01-01
• Subjects: Brown adipose tissue -- β-oxidation -- mitochondrial oxidative phosphorylation -- norepinephrine -- temperature reduction; Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.1080/13813455.2017.1360913
• Languages: English
• ISSNs: 1381-3455

Abstract

Abstract: Context: Heat generation by brown adipose tissue (BAT) in response to temperature reduction seems to be entirely related to sympathetic nervous stimulation. Objective: To analyse if temperature reduction and norepinephrine may differently affect the expression of proteins related to energy metabolism in BAT. Materials and methods: Isolated rats BAT was incubated with/without norepinephrine (10 –6 mol/L, 24 h at 32 °C and 37 °C). Results: In BAT, 32 °C increased the protein expression levels of carnitine palmitoyltransferase-I and -II, mitochondrial uncoupling protein-1 (UCP-1) and the expression and activity of lactate dehydrogenase. Mitochondrial F1 -ATP synthase α-chain expression was decreased at 32 °C compared to 37 °C. Norepinephrine and at 32 °C exposure, UCP-1 expression was increased but cytochrome-c oxidase and F1 -ATP synthase α-chain expression was reduced with respect to 37 °C. Discussion: Sympathetic stimulation seems not to be the only factor associated with heat generation. Conclusions: Temperature reduction by itself exerts some different effects on the expression of proteins related to the energy metabolism than norepinephrine.