1α, 25(OH)2D3 differentially regulates miRNA expression in human bladder cancer cells. Issue 148 (April 2015)

• Record Type: Journal Article
• Authors: Ma, Yingyu; Hu, Qiang; Luo, Wei; Pratt, Rachel N.; Glenn, Sean T.; Liu, Song; Trump, Donald L.; Johnson, Candace S.
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 148(2015)
• Page Start: 166
• Page End: 171
• Publication Date: 2015-04
• Subjects: CYP24 24-hydroxylase -- EtOH ethanol -- FBS fetal bovine serum -- miRNA microRNA -- PANTHER protein analysis through evolutionary relationships classification system -- TCC transitional cell carcinoma -- VDR vitamin D receptor; 1α, 25(OH)2D3 -- Bladder cancer -- miRNA -- Vitamin D; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2014.09.020
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: Human bladder cancer cells have functional vitamin D signaling. Distinct miRNA expression pattern observed in bladder cancer cells. 1α, 25(OH)2 D3 differentially regulate miRNA expression in bladder cancer cells. Abstract: Bladder cancer is the fourth most commonly diagnosed cancer in men and eighth leading cause of cancer-related death in the US. Epidemiological and experimental studies strongly suggest a role for 1α, 25(OH)2 D3 in cancer prevention and treatment. The antitumor activities of 1α, 25(OH)2 D3 are mediated by the induction of cell cycle arrest, apoptosis, differentiation and the inhibition of angiogenesis and metastasis. miRNAs play important regulatory roles in cancer development and progression. However, the role of 1α, 25(OH)2 D3 in the regulation of miRNA expression and the potential impact in bladder cancer has not been investigated. Therefore, we studied 1α, 25(OH)2 D3 -regulated miRNA expression profiles in human bladder cancer cell line 253J and the highly tumorigenic and metastatic derivative line 253J-BV by miRNA qPCR panels. 253J and 253J-BV cells express endogenous vitamin D receptor (VDR), which can be further induced by 1α, 25(OH)2 D3 . VDR target gene 24-hydroxylase was induced by 1α, 25(OH)2 D3 in both cell lines, indicating functional 1α, 25(OH)2 D3 signaling. The miRNA qPCR panel assay results showed that 253J and 253J-BV cells have distinct miRNA expression profiles. Further, 1α, 25(OH)2 D3 differentially regulated miRNA expression profiles in 253J and 253J-BV cells in a dynamic manner. Pathway analysis of the miRNA target genes revealed distinct patterns of contribution to the molecular functions and biological processes in the two cell lines. In conclusion, 1α, 25(OH)2 D3 differentially regulates the expression of miRNAs, which may contribute to distinct biological functions, in human bladder 253J and 253J-BV cells. This article is part of a Special Issue entitled '17th Vitamin D Workshop'.