The structure-function relationships of insulin-like growth factor 1 Ec in C2C12 cells. Issue 1 (2nd January 2018)

• Record Type: Journal Article
• Authors: Yi, Qian; Feng, Jianguo; He, Ling; Wan, Rongxue; Zeng, Hui; Yang, Li; Wu, Shiyong; Tang, Liling
• Is Part Of: Cell adhesion & migration. Volume 12:Issue 1(2018)
• Page Start: 47
• Page End: 55
• Publication Date: 2018-01-02
• Subjects: C2C12 cells -- differentiation -- IGF1Ec -- IGF1 -- IGF1–24 -- MGF E -- migration -- proliferation; Cell adhesion -- Periodicals; Cell migration -- Periodicals; Cell interaction -- Periodicals; 574.876
• DOI: 10.1080/19336918.2017.1318240
• Languages: English
• ISSNs: 1933-6918

Abstract

ABSTRACT: Insulin-like growth factor 1 (IGF1) is a crucial growth factor, that regulates skeletal muscles development during cell growth and repair. Recently, its alternative splicing variant, named IGF1Ec, also named mechano-growth factor (MGF), has gained attentions as a new damage repair factor. However, the structure-function relationships of IGF1Ec have not been fully clarified due to contradictory reports. In this study, we systematically investigated physiologic responses of C2C12 muscle cells to IGF1Ec, IGF1 and MGF E peptide. Our data indicate that while the N-terminal sequence of IGF1Ec, which is homolog in part with IGF1, promotes proliferation; the C-terminal sequence of IGF1Ec, which is identical to MGF E, promotes differentiation and migration of C2C12 cells. Our results suggest that MGF E cannot completely replace all the functions of IGF1Ec on muscle repair and regeneration, and elucidate the relationships between structure and function of IGF1Ec.