A novel combination of nano-scaffolds with micro-scaffolds to mimic extracellularmatrices improve osteogenesis. (July 2014)

• Record Type: Journal Article
• Authors: Xia, Yang; Peng, Sha-Sha; Xie, Li-Zhe; Lian, Xiao-Jie; Zhang, Xiao-Jun; Cui, Han; Song, Tian-Xi; Zhang, Fei-Min; Gu, Ning; Cui, Fu-Zhai
• Is Part Of: Journal of biomaterials applications. Volume 29:Number 1(2014:Jul.)
• Page Start: 59
• Page End: 71
• Publication Date: 2014-07
• Subjects: Peptide hydrogel -- nano-hydroxyapatite/collagen -- adipose-derived stem cells -- bone regeneration -- biocompatibility; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/0885328213514467
• Languages: English
• ISSNs: 0885-3282

Abstract

To improve bone engineering for clinical applications, we coupled nanofiber-peptide hydrogel to nano-hydroxyapatite/collagen to form a bioactive scaffold (cnHAC) that mimics extracellular matrices. In comparison to nano-hydroxyapatite/collagen, we found that cnHAC promoted cell adhesion and spreading, and DNA content measurements, alkaline phosphatase activity assays, and reverse transcriptase-polymerase chain reaction analyses of osteogenic gene expression showed that cnHAC significantly improved cellular attachment, proliferation, and osteogenic differentiation in vitro ( P < 0.05). In vivo models based on rat calvarial implants showed that cnHAC significantly enhanced bone regeneration ( P < 0.05). In conclusion, we demonstrated that novel cnHAC scaffolds could potentially facilitate future bone regenerative medicine.